Synthesis of alkaline protease modified ALPs-V2O5/MCM-41 nanocomposite and their multifunctional applications as an efficient photocatalytic, antibacterial and DPPH radicals stabilizing agent

Khalil ur Rehman, Imen Zghab, Mohammed Alissa, Kamran Tahir, Amira K. Hajri, Ferjeni Zouidi, Abdullah Alghamdi, Suad A. Alghamdi, Mohammed A. Alshehri, Alaa S. Alhegaili

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Background: The development of eco-friendly and sustainable nanomaterials is crucial for environmental safety and biomedical applications. This study presents a novel approach to synthesizing vanadium oxide-doped MCM-41 nanocomposites (V₂O₅/MCM-41) using an alkaline protease (ALPs) extracted from Phalaris minor seeds. Method: The ALPs-assisted synthesis method was employed for the first time to fabricate V₂O₅/MCM-41 nanocomposites, and their physicochemical properties were characterized to determine size, morphology, and crystallinity. Significant findings: The synthesized ALPs-V₂O₅/MCM-41 nanocomposites exhibited outstanding photocatalytic performance, achieving 97 % degradation of bromothymol blue (BTB) within 50 min of irradiation. Additionally, the synthesized nanocomposites exhibited strong antibacterial properties against Escherichia coli and Staphylococcus aureus, with inhibition zones measuring between 22(±0.3) mm and 31(±0.4) mm under both illuminated and non-illuminated conditions. Moreover, they exhibited remarkable antioxidant properties, with an 88 % scavenging efficacy against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals. Conclusion: These findings highlight the potential of ALPs-V₂O₅/MCM-41 nanocomposites as a sustainable and multifunctional material for environmental remediation and antimicrobial applications, paving the way for further research in eco-friendly nanotechnology.

Original languageEnglish
Article number106136
JournalJournal of the Taiwan Institute of Chemical Engineers
Volume172
DOIs
StatePublished - Jul 2025

Keywords

  • ALPs-VO/MCM-41 nanocomposite
  • Alkaline protease
  • Photocatalytic activity

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