Sulfation of chitosan from Sepia kobiensis as potential anticoagulant and antibacterial molecule

The present work aimed to synthesis of chitin, chitosan and sulfation of chitosan from cuttlebone of cuttlefish Sepia kobiensis. Principally chitin was extracted through sequential processes of demineralisation and deproteinzation. Then chitosan was synthesized by a deacetylation and finally sulfated at semi-heterogeneous condition using chlorosulfonic acid in N,N-dimethylformamide. The synthesized macromolecules were characterized for its structural, physical and thermal (CHN, DDA, FT-IR, NMR, XRD, Viscometric analysis, SEM and DSC) properties. Apart from anticoagulant potential of the sulfated chitosan was tested using human plasma by means of activated partial thromboplastin time (APTT) and prothrombin time (PT). Further sulfated chitosan was tested for antibacterial potential by well diffusion method against eleven human pathogenic clinical isolates of both Gram positive and Gram-negative strains and minimum inhibitory concentrations (MIC) was calculated accordingly. The results of this study revealed the effectiveness of the sulfated chitosan at semi-heterogeneous conditions as a potent antibacterial and anticoagulant molecule.