TY - JOUR
T1 - Standardization and antioxidant studies of arnebia hispidissima
AU - Soliman Yusufoglu, Hasan
AU - Soliman, Gamal A.
AU - Foudah, Ahmed I.
AU - Abdulkader, Maged S.
AU - Alqarni, Mohammed H.
AU - Alam, Aftab
AU - Salkini, Mohammad Ayman
N1 - Publisher Copyright:
© 2018 Hasan S. Yusufoglu et al.
PY - 2018
Y1 - 2018
N2 - Background and Objective: Arnebia hispidissima (A. hispidissima) a member of the family Boraginaceae, is dye yielding and medicinally important plant. It is widely used in the cosmetic industries. This study has been made for the preliminary standardization of A. hispidissima plant. Methodology: The standardization evaluation comprises of powder microscopy and fluorescence analysis and TLC profiling. In addition, preliminary phytochemical screening, determination of total phenol and in vitro free radical scavenging activity (DPPH radicals scavenging assay and reducing power activity) were preformed utilizing the methanol extract. Results: A microscopic study of powder of whole plant of A. hispidissima showed different types of trichomes, vessel and fibres. Fluorescence analysis showed different colours under visible light, low UV and high UV. TLC of the hexane extract developed 8, 6, 9 and 10 spots with visible light, low UV, high UV and ninhydrin-H2SO4 spray, respectively. The phytochemical analysis of the methanol extract gave a positive indication for the presence of active compounds including alkaloids, carbohydrates, glycosides, steroids, triterpenoids saponins, phenols, tannins, flavonoids and proteins. The quantitative analysis showed the presence of a significant quantity of total phenol and the in vitro antioxidant activity clearly showed the terrific antioxidant property. Conclusion: The data generated from the present study can be utilized for the identification and quality control of A. hispidissima plant.
AB - Background and Objective: Arnebia hispidissima (A. hispidissima) a member of the family Boraginaceae, is dye yielding and medicinally important plant. It is widely used in the cosmetic industries. This study has been made for the preliminary standardization of A. hispidissima plant. Methodology: The standardization evaluation comprises of powder microscopy and fluorescence analysis and TLC profiling. In addition, preliminary phytochemical screening, determination of total phenol and in vitro free radical scavenging activity (DPPH radicals scavenging assay and reducing power activity) were preformed utilizing the methanol extract. Results: A microscopic study of powder of whole plant of A. hispidissima showed different types of trichomes, vessel and fibres. Fluorescence analysis showed different colours under visible light, low UV and high UV. TLC of the hexane extract developed 8, 6, 9 and 10 spots with visible light, low UV, high UV and ninhydrin-H2SO4 spray, respectively. The phytochemical analysis of the methanol extract gave a positive indication for the presence of active compounds including alkaloids, carbohydrates, glycosides, steroids, triterpenoids saponins, phenols, tannins, flavonoids and proteins. The quantitative analysis showed the presence of a significant quantity of total phenol and the in vitro antioxidant activity clearly showed the terrific antioxidant property. Conclusion: The data generated from the present study can be utilized for the identification and quality control of A. hispidissima plant.
KW - Arnebia hispidissima
KW - Chromatography
KW - DPPH
KW - Microscopical
KW - Phytochemical
KW - Reducing power
UR - http://www.scopus.com/inward/record.url?scp=85044931251&partnerID=8YFLogxK
U2 - 10.3923/ijp.2018.428.436
DO - 10.3923/ijp.2018.428.436
M3 - Article
AN - SCOPUS:85044931251
SN - 1811-7775
VL - 14
SP - 428
EP - 436
JO - International Journal of Pharmacology
JF - International Journal of Pharmacology
IS - 3
ER -