Stability-indicating densitometric HPTLC method for qualitative and quantitative analysis of hydroquinone in commercial whitening creams

Saleh I. Alqasoumi; Prawez Alam; Adnan J. Al-Rehaily; Faiyaz Shakeel; Maged S. Abdel-Kader

doi:10.1556/JPC.24.2011.1.9

Stability-indicating densitometric HPTLC method for qualitative and quantitative analysis of hydroquinone in commercial whitening creams

Saleh I. Alqasoumi, Prawez Alam, Adnan J. Al-Rehaily, Faiyaz Shakeel, Maged S. Abdel-Kader

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10 Scopus citations

Abstract

A densitometric high-performance thin-layer chromatographic (HPTLC) method for analysis of hydroquinone has been developed and validated. Chromatography was performed on aluminum foilbacked silica gel 60 F₂₅₄ plates with chloroform-methanol 85:15 (% v/v) as mobile phase. This system furnished a compact band for hydroquinone at R_F 0.51. Hydroquinone was quantified densitometrically at 289 nm. The limits of detection (LOD) and quantification (LOQ) were 38.50 and 115.50 ng per band, respectively. High precision and accuracy were achieved. The method was used for both qualitative and quantitative analysis of hydroquinone in commercial formulations. Because the method can effectively separate the hydroquinone in the presence of its degradation products, it can be used as a stability-indicating method.

Original language	English
Pages (from-to)	48-52
Number of pages	5
Journal	Journal of Planar Chromatography - Modern TLC
Volume	24
Issue number	1
DOIs	https://doi.org/10.1556/JPC.24.2011.1.9
State	Published - 1 Feb 2011

Keywords

HPTLC
Hydroquinone
ICH guidelines
Qualitative
Quantitative
Whitening creams

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10.1556/JPC.24.2011.1.9

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title = "Stability-indicating densitometric HPTLC method for qualitative and quantitative analysis of hydroquinone in commercial whitening creams",

abstract = "A densitometric high-performance thin-layer chromatographic (HPTLC) method for analysis of hydroquinone has been developed and validated. Chromatography was performed on aluminum foilbacked silica gel 60 F254 plates with chloroform-methanol 85:15 (\% v/v) as mobile phase. This system furnished a compact band for hydroquinone at RF 0.51. Hydroquinone was quantified densitometrically at 289 nm. The limits of detection (LOD) and quantification (LOQ) were 38.50 and 115.50 ng per band, respectively. High precision and accuracy were achieved. The method was used for both qualitative and quantitative analysis of hydroquinone in commercial formulations. Because the method can effectively separate the hydroquinone in the presence of its degradation products, it can be used as a stability-indicating method.",

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AU - Alqasoumi, Saleh I.

AU - Alam, Prawez

AU - Al-Rehaily, Adnan J.

AU - Shakeel, Faiyaz

AU - Abdel-Kader, Maged S.

PY - 2011/2/1

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AB - A densitometric high-performance thin-layer chromatographic (HPTLC) method for analysis of hydroquinone has been developed and validated. Chromatography was performed on aluminum foilbacked silica gel 60 F254 plates with chloroform-methanol 85:15 (% v/v) as mobile phase. This system furnished a compact band for hydroquinone at RF 0.51. Hydroquinone was quantified densitometrically at 289 nm. The limits of detection (LOD) and quantification (LOQ) were 38.50 and 115.50 ng per band, respectively. High precision and accuracy were achieved. The method was used for both qualitative and quantitative analysis of hydroquinone in commercial formulations. Because the method can effectively separate the hydroquinone in the presence of its degradation products, it can be used as a stability-indicating method.

KW - HPTLC

KW - Hydroquinone

KW - ICH guidelines

KW - Qualitative

KW - Quantitative

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Stability-indicating densitometric HPTLC method for qualitative and quantitative analysis of hydroquinone in commercial whitening creams

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Keywords

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