TY - JOUR
T1 - Sol-gel synthesized rutile TiO2 nanoparticles loaded with cardamom essential oil
T2 - Enhanced antibacterial activity
AU - Ouerghi, Oussama
AU - Geesi, Mohammed H.
AU - Ibnouf, Elmutasim O.
AU - Ansari, Mohammad Javed
AU - Alam, Pravej
AU - Elsanousi, Ammar
AU - Kaiba, Abdellah
AU - Riadi, Yassine
N1 - Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2021/8
Y1 - 2021/8
N2 - Nanoparticles have been recognized as a promising alternative to overcome problems associated with antibiotic-resistant bacterial strains. In the current study, the combination of TiO2 nanoparticles with cardamom essential oil (CEO) was assessed for its antibacterial effects. First, TiO2 nanoparticles were synthesized via sol-gel route and subjected to various physico-chemical characterizations; structural using both X-ray diffraction and Raman spectroscopy, morphological by scanning electron microscopy and elemental analysis by X-ray photoelectron spectroscopy. The synthesized nanoparticles were, then, loaded with natural CEO to form the conjugate CEO@TiO2. The effectiveness of the conjugate CEO@TiO2 was assessed against a standard test strain as well as against two clinical isolates using ultraviolet–visible spectrophotometry measurements. The results demonstrate that CEO@TiO2 conjugates exhibit potent antibacterial activity against all the tested bacteria than CEO or TiO2 nanoparticles alone. An attempt to explain the mechanism of action of the devised antibacterial agent was undertaken based on confocal microscopy observations. Overall, this proof-of-concept antibacterial system may have important implications for the development of numerous therapeutic, environmental and food industry applications.
AB - Nanoparticles have been recognized as a promising alternative to overcome problems associated with antibiotic-resistant bacterial strains. In the current study, the combination of TiO2 nanoparticles with cardamom essential oil (CEO) was assessed for its antibacterial effects. First, TiO2 nanoparticles were synthesized via sol-gel route and subjected to various physico-chemical characterizations; structural using both X-ray diffraction and Raman spectroscopy, morphological by scanning electron microscopy and elemental analysis by X-ray photoelectron spectroscopy. The synthesized nanoparticles were, then, loaded with natural CEO to form the conjugate CEO@TiO2. The effectiveness of the conjugate CEO@TiO2 was assessed against a standard test strain as well as against two clinical isolates using ultraviolet–visible spectrophotometry measurements. The results demonstrate that CEO@TiO2 conjugates exhibit potent antibacterial activity against all the tested bacteria than CEO or TiO2 nanoparticles alone. An attempt to explain the mechanism of action of the devised antibacterial agent was undertaken based on confocal microscopy observations. Overall, this proof-of-concept antibacterial system may have important implications for the development of numerous therapeutic, environmental and food industry applications.
KW - Antibacterial effect
KW - Cardamom essential oil
KW - Rutile TiO nanoparticles
KW - Sol-gel synthesis
UR - http://www.scopus.com/inward/record.url?scp=85107539716&partnerID=8YFLogxK
U2 - 10.1016/j.jddst.2021.102581
DO - 10.1016/j.jddst.2021.102581
M3 - Article
AN - SCOPUS:85107539716
SN - 1773-2247
VL - 64
JO - Journal of Drug Delivery Science and Technology
JF - Journal of Drug Delivery Science and Technology
M1 - 102581
ER -