Simultaneous quantification of mefenamic acid and paracetamol in fixed-dose combination tablet dosage forms using the green HPTLC method

In this study, a green reversed-phase "high-performance thin-layer chromatography (HPTLC)"technique is established and verified for the detection of mefenamic acid (MEF) and paracetamol (PCM) simultaneously in their fixed-dose combination tablets. MEF and PCM were measured simultaneously using a green developing system consisting of ethanol, water, and glacial acetic acid in a 75:23:2 (v/v/v) ternary ratio. Both MEF and PCM were concurrently identified at a wavelength of 235 nm. Three distinct greenness methodologies - analytical eco-scale (AES), chloroform toxicity (ChlorTox), and analytical GREEnness (AGREE) - were used to assess the method's greenness profile. For both medications, the devised technique was linear in the 25-800 ng·band^-1 range. After validation, the devised approach was found to be accurate, precise, sensitive, robust, and environmentally friendly. Each of the greenness tools' results, including those from AGREE (0.82), ChlorTox (0.82 g), and AES (91), showed that the current approach had a noticeably greener profile. Using the current method, it was determined that the MEF and PCM levels in commercial fixed-dose combination tablet brands A and B were within the 100 ± 2% limit. The results of investigation showed that MEF and PCM in commercial combination tablets could be reliably analyzed using the recommended method.