RP-HPLC method for the analysis of quercetin in Eruca Sativa with green solvent

Background: Quercetin is a major representative of flavonol, sub-classs of flavonoids which has a nutritional value and active moieties of many medicinal plants with potent antioxidant, Anti-diabetic, anti-tumor, antiviral and anti-inflammatory properties. Eruca Sativa one of the most consumable herbs in Saudi Arabia, has not been analyzed yet by using green solvent. Objectives: A simple, sensitive, accurate, and precise reversed phase liquid chromatography method was developed and validated for the quantitative estimation of flavonoid quercetin from the extract of Eruca Sativa. Method: The flavonoid was analyzed on RP-HPLC using Lichrosphere-100, C₁₈, column (25cm × 4.6mm ID, 5μm) with UV detector system. The mobile phase consisting Methanol and water having β-cyclodextrin (5mM) with 0.1 % orthophosphoric acid(70: 30 v/v) solution was used with flow rate of 1.0 mL min^-1 and detection was performed at 370 nm wavelength. Results: The data showed that the linear ranges of Quercetin were 02-14 μg mL^-1 (r=0.997), intraday and intermediate precision at three concentration level as % RSD (0.4550,0.7251, 0.5075; 0.4859,0.7999,0.5618), accuracy at three concentration level as % recovery (102.3 ± 0.8074,101.17± 0.9074,101.46 ± 0.1856), limit of detection; LOD (3.64042E-05μg mL^-1) and limit of quantitation, LOQ (0.00011μg mL^-1) and robustness (in significant variations expressed as % RSD 0.13). Conclusion: The method was applied to quantitative analysis of Quercetin in plant extract of Eruca Sativa and was found to be simple, rapid and efficient.