Phosphatidylserine induced up-regulation of cluster differentiation 36 and 47 on red cell membrane

Aim: The purpose of this research was to study the expression of CD36 and CD47 on red cell membrane following perturbation of red cell membrane with phosphtidylserine (PS). Materials and Methods: Blood was drawn from 100 healthy adults; Incubation of RBCs with different serial concentrations of PS was carried out at 37°C for 72 hrs. All tubes were washed followed by addition of CD36-FITC and CD47-FITC antibodies, Cells were analyzed for the expression of CD markers using the FACS analyzer. Data were analyzed by using Statistical Package for Social Science (SPSS) version 21 and Microsoft Excel 2013. Results: were obtained by using student T.test. Result: CD36 expression is directly proportional to PS serial dilutions; however the expression of CD47 is raised in the last three higher concentrations. Conclusion: We conclude that CD47 has been shown to inhibit phagocytosis of erythrocytes by macrophages of the reticuloendothelial system, whilst CD36 responsible of recognition of red cell by macrophage and accelerating engulfing process PS induced up regulation of CD36 and CD47 which may encourage their conversed action and participate in keeping red cell as much as normal in diseases associated with high concentration of PS.