TY - JOUR
T1 - Olopatadine enhances recovery of alkali-induced corneal injury in rats
AU - Kandeel, Samah
AU - Balaha, Mohamed
N1 - Publisher Copyright:
© 2018 Elsevier Inc.
PY - 2018/8/15
Y1 - 2018/8/15
N2 - Aims: The alkali-induced corneal injury is an ocular emergency that required an immediate and effective management to preserve the normal corneal functions and transparency. Olopatadine is a fast, topically-effective anti-allergic drug, which exhibited potent anti-inflammatory and anti-angiogenic abilities in different allergic animals’ models. Therefore, this study aimed to evaluate the therapeutic effect of olopatadine on alkali-induced corneal injury in rats. Materials and methods: Corneal alkali injury (CI) induced in the right eyes of an eight-week-old male Wister rats, by application of 3 mm diameter filter-papers, soaked for 10 s in 1 N-NaOH, to the right eyes’ corneal centers for 30 s, afterward, the filter paper removed, and the rat right eye rinsed with 20 ml normal saline. For treatment of CI, either 0.2% or 0.77% olopatadine applied topically daily for 14 days, starting immediately after the induction of CI. Key findings: Olopatadine, in the present work, effectively and dose-dependently enhanced the corneal healing after alkali application, with significant reduction of the corneal opacity and neovascularization scores, besides, it suppressed the augmented corneal IL-1β VEGF, caspase-3 levels, and nuclear NF-κB immunohistochemical expression, meanwhile it abrogated the corneal histopathological changes, induced by alkali application. Significance: Olopatadine appears to be a potential treatment option for alkali-induced corneal injury.
AB - Aims: The alkali-induced corneal injury is an ocular emergency that required an immediate and effective management to preserve the normal corneal functions and transparency. Olopatadine is a fast, topically-effective anti-allergic drug, which exhibited potent anti-inflammatory and anti-angiogenic abilities in different allergic animals’ models. Therefore, this study aimed to evaluate the therapeutic effect of olopatadine on alkali-induced corneal injury in rats. Materials and methods: Corneal alkali injury (CI) induced in the right eyes of an eight-week-old male Wister rats, by application of 3 mm diameter filter-papers, soaked for 10 s in 1 N-NaOH, to the right eyes’ corneal centers for 30 s, afterward, the filter paper removed, and the rat right eye rinsed with 20 ml normal saline. For treatment of CI, either 0.2% or 0.77% olopatadine applied topically daily for 14 days, starting immediately after the induction of CI. Key findings: Olopatadine, in the present work, effectively and dose-dependently enhanced the corneal healing after alkali application, with significant reduction of the corneal opacity and neovascularization scores, besides, it suppressed the augmented corneal IL-1β VEGF, caspase-3 levels, and nuclear NF-κB immunohistochemical expression, meanwhile it abrogated the corneal histopathological changes, induced by alkali application. Significance: Olopatadine appears to be a potential treatment option for alkali-induced corneal injury.
KW - Alkali
KW - Caspase-3
KW - Cornea
KW - IL-1β
KW - NF-κB expression
KW - VEGF
UR - http://www.scopus.com/inward/record.url?scp=85049485513&partnerID=8YFLogxK
U2 - 10.1016/j.lfs.2018.07.002
DO - 10.1016/j.lfs.2018.07.002
M3 - Article
C2 - 30056863
AN - SCOPUS:85049485513
SN - 0024-3205
VL - 207
SP - 499
EP - 507
JO - Life Sciences
JF - Life Sciences
ER -