TY - JOUR
T1 - Novel HPTLC-densitometric method for concurrent quantification of linalool and thymol in essential oils
AU - Alqarni, Mohammad H.
AU - Foudah, Ahmed I.
AU - Alam, Aftab
AU - Salkini, Mohammad Ayman
AU - Alam, Prawez
AU - Soliman Yusufoglu, Hasan
N1 - Publisher Copyright:
© 2020 The Author(s)
PY - 2021/2
Y1 - 2021/2
N2 - Monoterpenes, linalool and thymol have been widely used in not only the treatments of several diseases but also food flavouring, dental, perfumery, and toilet products. Plants of the several Lamiaceae family are good sources of linalool and thymol. A rapid, simple, and precise high-performance thin layer chromatography (HPTLC) method was developed for the concurrent estimation of linalool and thymol, and this method was orchestrated using the essential oils extracted from two Lamiaceae plants leaves i.e. O. basilicum and T. vulgaris. Two standards were separated using pre-coated thin-layer chromatography glass plates. The developed HPTLC method was validated by following ICH guidelines (linearity; limit of detection, LOD; limit of quantitation; specificity; accuracy; precision; and robustness). The calibration curves of both the compounds were linear (100–700 ng/spot), with a correlation coefficient (r2) of >999. The developed HPTLC method was effectively applied to the concurrent detection and quantification of linalool and thymol in commercial essential oils.
AB - Monoterpenes, linalool and thymol have been widely used in not only the treatments of several diseases but also food flavouring, dental, perfumery, and toilet products. Plants of the several Lamiaceae family are good sources of linalool and thymol. A rapid, simple, and precise high-performance thin layer chromatography (HPTLC) method was developed for the concurrent estimation of linalool and thymol, and this method was orchestrated using the essential oils extracted from two Lamiaceae plants leaves i.e. O. basilicum and T. vulgaris. Two standards were separated using pre-coated thin-layer chromatography glass plates. The developed HPTLC method was validated by following ICH guidelines (linearity; limit of detection, LOD; limit of quantitation; specificity; accuracy; precision; and robustness). The calibration curves of both the compounds were linear (100–700 ng/spot), with a correlation coefficient (r2) of >999. The developed HPTLC method was effectively applied to the concurrent detection and quantification of linalool and thymol in commercial essential oils.
KW - HPTLC
KW - Linalool
KW - Method development
KW - Ocimum basilicum
KW - Thymol
KW - Thymus vulgaris
UR - http://www.scopus.com/inward/record.url?scp=85098452997&partnerID=8YFLogxK
U2 - 10.1016/j.arabjc.2020.102916
DO - 10.1016/j.arabjc.2020.102916
M3 - Article
AN - SCOPUS:85098452997
SN - 1878-5352
VL - 14
JO - Arabian Journal of Chemistry
JF - Arabian Journal of Chemistry
IS - 2
M1 - 102916
ER -