Abstract
Candida albicans is a widespread opportunistic pathogen in immunocompromised patients. ATPase activity and protein concentration using spectrophotometric method and antifungal activity by Microtitre/ELISA technique in terms of Minimum Inhibitory Concentration (MIC) was studied to understand the pattern of Candida albicans. Minimum Inhibitory Concentration for Clotrimazole and Nystatin showed 6.25μg/ml and 8.0μg/ml "respectively". At optimum pH (7.0) and temperature (28°C), 100% activity of ATPase was recorded. pH showed inverse relationship while temperature showed no remarkable variation of activity. ATPase was found to be 5.0% reduced by Clotrimazole, whereas Nystatin caused 39% inhibition at the optimum pH and temperature.
| Original language | English |
|---|---|
| Pages (from-to) | 4149-4154 |
| Number of pages | 6 |
| Journal | Journal of Pure and Applied Microbiology |
| Volume | 8 |
| Issue number | 5 |
| State | Published - 1 Oct 2014 |
Keywords
- ATPase
- Candida albicans
- Candidiasis
- Nystatin and Clotrimazole