Mesenchymal stem cells versus mesenchymal stem cells-derived exosomes as potential autophagy pathway modulators in a diabetic model

Fatma Y. Meligy; Hanan Sharaf El Deen Mohammed; Amal T. Abou Elghait; Heba K. Mohamed; Israa El Sayed Mohamed Ashry; Ayat Abdel-Rahman Sayed; Ola A. Hussein; Ahmed Salman; Tarek Atia; Abir S. Mohamed; Nour H. Behnsawy; Safy Salah Gaber; Hader I. Sakr; Salwa Fares Ahmed

doi:10.1016/j.advms.2025.02.004

Mesenchymal stem cells versus mesenchymal stem cells-derived exosomes as potential autophagy pathway modulators in a diabetic model

Fatma Y. Meligy
, Hanan Sharaf El Deen Mohammed
, Amal T. Abou Elghait
, Heba K. Mohamed
, Israa El Sayed Mohamed Ashry
, Ayat Abdel-Rahman Sayed
, Ola A. Hussein
, Ahmed Salman
, Tarek Atia
, Abir S. Mohamed
, Nour H. Behnsawy
, Safy Salah Gaber
, Hader I. Sakr
, Salwa Fares Ahmed

Medical Laboratory Sciences

University of Petra
Assiut University
Sphinx University
University of Jordan
Menoufia University
Jazan University
International Federation of Medical Students Association
Beni-Suef University
Cairo University
Batterjee Medical College
University of Tabuk

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Purpose: This work compared the potential effects of bone marrow mesenchymal stem cells (BM-MSCs) with BM-MSCs-derived exosomes against impaired autophagy in streptozotocin (STZ)-induced diabetic rats. Materials and methods: Three days after STZ injection, a single dose of (3 × 10^6) BM- MSCs or BM-MSCs-derived exosomes (80 μg/rat) was administered to evaluate their effects against nondiabetic and diabetic control rats. We assessed pancreatic structure via light and electron microscopy and evaluated its staining for insulin and the autophagy marker P62 immunohistochemically. Moreover, autophagy marker LC3 gene expression was examined by PCR. Results: Both BM-MSCs and BM-MSCs derived exosomes showed histological restoration of pancreatic tissues. Both treatments markedly increased the amount of insulin and significantly decreased the autophagy markers P62 and LC3. Conclusion: Our findings suggest that both BM-MSCs and BM-MSCs-derived exosomes provides a potential alternative to modulate diabetes mellitus.

Original language	English
Pages (from-to)	152-165
Number of pages	14
Journal	Advances in Medical Sciences
Volume	70
Issue number	1
DOIs	https://doi.org/10.1016/j.advms.2025.02.004
State	Published - Mar 2025

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Autophagy
Diabetes mellitus
Exosome
Mesenchymal stem cells

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10.1016/j.advms.2025.02.004

Cite this

Meligy, F. Y., Mohammed, H. S. E. D., Abou Elghait, A. T., Mohamed, H. K., Ashry, I. E. S. M., Abdel-Rahman Sayed, A., Hussein, O. A., Salman, A., Atia, T., Mohamed, A. S., Behnsawy, N. H., Gaber, S. S., Sakr, H. I., & Ahmed, S. F. (2025). Mesenchymal stem cells versus mesenchymal stem cells-derived exosomes as potential autophagy pathway modulators in a diabetic model. Advances in Medical Sciences, 70(1), 152-165. https://doi.org/10.1016/j.advms.2025.02.004

@article{810e7f61667448c8a93ee35bcd4e5c04,

title = "Mesenchymal stem cells versus mesenchymal stem cells-derived exosomes as potential autophagy pathway modulators in a diabetic model",

abstract = "Purpose: This work compared the potential effects of bone marrow mesenchymal stem cells (BM-MSCs) with BM-MSCs-derived exosomes against impaired autophagy in streptozotocin (STZ)-induced diabetic rats. Materials and methods: Three days after STZ injection, a single dose of (3 × 10\textasciicircum{}6) BM- MSCs or BM-MSCs-derived exosomes (80 μg/rat) was administered to evaluate their effects against nondiabetic and diabetic control rats. We assessed pancreatic structure via light and electron microscopy and evaluated its staining for insulin and the autophagy marker P62 immunohistochemically. Moreover, autophagy marker LC3 gene expression was examined by PCR. Results: Both BM-MSCs and BM-MSCs derived exosomes showed histological restoration of pancreatic tissues. Both treatments markedly increased the amount of insulin and significantly decreased the autophagy markers P62 and LC3. Conclusion: Our findings suggest that both BM-MSCs and BM-MSCs-derived exosomes provides a potential alternative to modulate diabetes mellitus.",

keywords = "Autophagy, Diabetes mellitus, Exosome, Mesenchymal stem cells",

author = "Meligy, \{Fatma Y.\} and Mohammed, \{Hanan Sharaf El Deen\} and \{Abou Elghait\}, \{Amal T.\} and Mohamed, \{Heba K.\} and Ashry, \{Israa El Sayed Mohamed\} and \{Abdel-Rahman Sayed\}, Ayat and Hussein, \{Ola A.\} and Ahmed Salman and Tarek Atia and Mohamed, \{Abir S.\} and Behnsawy, \{Nour H.\} and Gaber, \{Safy Salah\} and Sakr, \{Hader I.\} and Ahmed, \{Salwa Fares\}",

note = "Publisher Copyright: {\textcopyright} 2025 The Authors",

year = "2025",

month = mar,

doi = "10.1016/j.advms.2025.02.004",

language = "English",

volume = "70",

pages = "152--165",

journal = "Advances in Medical Sciences",

issn = "1896-1126",

publisher = "Medical University of Bialystok",

number = "1",

}

Meligy, FY, Mohammed, HSED, Abou Elghait, AT, Mohamed, HK, Ashry, IESM, Abdel-Rahman Sayed, A, Hussein, OA, Salman, A, Atia, T, Mohamed, AS, Behnsawy, NH, Gaber, SS, Sakr, HI & Ahmed, SF 2025, 'Mesenchymal stem cells versus mesenchymal stem cells-derived exosomes as potential autophagy pathway modulators in a diabetic model', Advances in Medical Sciences, vol. 70, no. 1, pp. 152-165. https://doi.org/10.1016/j.advms.2025.02.004

TY - JOUR

T1 - Mesenchymal stem cells versus mesenchymal stem cells-derived exosomes as potential autophagy pathway modulators in a diabetic model

AU - Meligy, Fatma Y.

AU - Mohammed, Hanan Sharaf El Deen

AU - Abou Elghait, Amal T.

AU - Mohamed, Heba K.

AU - Ashry, Israa El Sayed Mohamed

AU - Abdel-Rahman Sayed, Ayat

AU - Hussein, Ola A.

AU - Salman, Ahmed

AU - Atia, Tarek

AU - Mohamed, Abir S.

AU - Behnsawy, Nour H.

AU - Gaber, Safy Salah

AU - Sakr, Hader I.

AU - Ahmed, Salwa Fares

PY - 2025/3

Y1 - 2025/3

N2 - Purpose: This work compared the potential effects of bone marrow mesenchymal stem cells (BM-MSCs) with BM-MSCs-derived exosomes against impaired autophagy in streptozotocin (STZ)-induced diabetic rats. Materials and methods: Three days after STZ injection, a single dose of (3 × 10^6) BM- MSCs or BM-MSCs-derived exosomes (80 μg/rat) was administered to evaluate their effects against nondiabetic and diabetic control rats. We assessed pancreatic structure via light and electron microscopy and evaluated its staining for insulin and the autophagy marker P62 immunohistochemically. Moreover, autophagy marker LC3 gene expression was examined by PCR. Results: Both BM-MSCs and BM-MSCs derived exosomes showed histological restoration of pancreatic tissues. Both treatments markedly increased the amount of insulin and significantly decreased the autophagy markers P62 and LC3. Conclusion: Our findings suggest that both BM-MSCs and BM-MSCs-derived exosomes provides a potential alternative to modulate diabetes mellitus.

AB - Purpose: This work compared the potential effects of bone marrow mesenchymal stem cells (BM-MSCs) with BM-MSCs-derived exosomes against impaired autophagy in streptozotocin (STZ)-induced diabetic rats. Materials and methods: Three days after STZ injection, a single dose of (3 × 10^6) BM- MSCs or BM-MSCs-derived exosomes (80 μg/rat) was administered to evaluate their effects against nondiabetic and diabetic control rats. We assessed pancreatic structure via light and electron microscopy and evaluated its staining for insulin and the autophagy marker P62 immunohistochemically. Moreover, autophagy marker LC3 gene expression was examined by PCR. Results: Both BM-MSCs and BM-MSCs derived exosomes showed histological restoration of pancreatic tissues. Both treatments markedly increased the amount of insulin and significantly decreased the autophagy markers P62 and LC3. Conclusion: Our findings suggest that both BM-MSCs and BM-MSCs-derived exosomes provides a potential alternative to modulate diabetes mellitus.

KW - Autophagy

KW - Diabetes mellitus

KW - Exosome

KW - Mesenchymal stem cells

UR - https://www.scopus.com/pages/publications/85217943562

U2 - 10.1016/j.advms.2025.02.004

DO - 10.1016/j.advms.2025.02.004

M3 - Article

C2 - 39956208

AN - SCOPUS:85217943562

SN - 1896-1126

VL - 70

SP - 152

EP - 165

JO - Advances in Medical Sciences

JF - Advances in Medical Sciences

IS - 1

ER -

Mesenchymal stem cells versus mesenchymal stem cells-derived exosomes as potential autophagy pathway modulators in a diabetic model

Abstract

UN SDGs

Keywords

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