Mechanistic understanding of Ptyrone^TM: A plant based natural anti diabetic product

Objective: To investigate the combinatorial effect of hydroalcoholic extracts of Andrographis paniculata Nees. and Pterocarpus marsupium Roxb. plants prospective to diabetes management. Methods: Taking a lead from the scientific literature, in silico studies have also been designed for the screening of anti-diabetic targets against andrographolide and pterostilbene compounds followed by in vivo studies from Andrographis paniculata Nees. and Pterocarpus marsupium Roxb. Furthermore, the diabetes was induced by STZ model and the impact of Andrographis paniculata Nees. and Pterocarpus marsupium Roxb. have been conformed by relative expression studies by qPCR. Results: Our results have shown that andrographolide and pterostilbene are SGLT2 inhibitors and selective PPARγ agonists in in silico studies. Later, during in vivo mRNA expression studies confirming the same pattern. The findings of the study has shown to overcome the common knowledge of the only C–glycoside based molecules inhibiting the SGLT2. Conclusions: The possible mechanism for Ptyrone^TM in the management of diabetes could be a selective PPARγ agonist, GLUT4 translocation and SGLT2 inhibition molecule.