Immobilization of phytase on chitosan-coated iron oxide nanoparticles (Fe₃O₄ NPs) and its application for the reduction of soymilk phytic acid

In this study, phytase from Chenopodium album was isolated, characterized, and immobilized using nano-Fe₃O₄ nanoparticles on the surface of modified chitosan for reduction of phytic acid in soymilk. The phytase was purified 120.2-fold and yielded 11.6 % of the sample within a 40–80 % range. The SDS-PAGE was employed to calculate the molecular weight (50 kDa) of the phytase. Some surface analysis methods, including SEM, XRD, and FTIR analysis were applied to inquire immobilized phytase. Some natural polymers, including, starch cellulose, phytate, locust bean xylene, gum, and gelatin, were among the natural polymers used to calculate the substrate specificity of the purified enzyme. For over seven batch reactions, operational reusability was studied, and storage stability retained 70 % of its catalytic activity for 40 days. The reduction of phytic acid in soymilk can be done easily and efficiently with the help of the current process, which has a higher prospective in plausible applications.