Abstract
Objective: To develop a sensitive and accurate high-performance thin layer chromatography method and to determine the quantity of thymoquinone in in two different N. sativa extracts and marketed formulations. Methods: Thymoquinone was seperated on aluminum-backed silica gel 60 F254 plates with n-hexane-ethyl acetate 8:2 (%, v/v) as mobile phase. Results: A compact band was obtained for thymoquinone at Rf value of 0.48±0.04. The calibration plot was linear in the range of 50-700 ng/spot of thymoquinone and the correlation coefficient of 0.9989 was indicative of good linear dependence of peak area on concentration. Limit of detection (8.67 ng/spot), limit of quantification (17.43 ng/spot) accuracy (less than 2) and recovery (ranging from 98.39-99.17) were found satisfactory. Conclusions: The developed high-performance thin layer chromatography densitometric method was found cheap, selective, precise and accurate and can be used for routine analysis of N. sativa extracts and marketed formulations.
| Original language | English |
|---|---|
| Pages (from-to) | 467-471 |
| Number of pages | 5 |
| Journal | Asian Pacific Journal of Tropical Disease |
| Volume | 3 |
| Issue number | 6 |
| DOIs | |
| State | Published - Dec 2013 |
Keywords
- HPTLC densitometry
- ICH guidelines
- Qualitative
- Quantitative
- Thymoquinone