Hematopoietic effect of echinochrome on phenylhydrazine-induced hemolytic anemia in rats

Mona S.E.F. El-Shehry; Rafa A. Amrymi; Tarek Atia; Bassant M.M. Lotfy; Salma H.A. Ahmed; Sarah A. Qutb; Sara B. Ali; Ayman S. Mohamed; Mohamed R. Mousa; Ahmed A. Damanhory; Mostafa E. Metawee; Hader I. Sakr

doi:10.7717/peerj.16576

Hematopoietic effect of echinochrome on phenylhydrazine-induced hemolytic anemia in rats

Mona S.E.F. El-Shehry, Rafa A. Amrymi, Tarek Atia, Bassant M.M. Lotfy, Salma H.A. Ahmed, Sarah A. Qutb, Sara B. Ali, Ayman S. Mohamed, Mohamed R. Mousa, Ahmed A. Damanhory, Mostafa E. Metawee, Hader I. Sakr

Medical Laboratory Sciences

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Background: Hemolytic anemia (HA) is a serious health condition resulting from reduced erythrocytes’ average life span. Echinochrome (Ech) is a dark-red pigment found in shells and spines of sea urchins. Aim: Studying the potential therapeutic effect of Ech on phenylhydrazine (PHZ)induced HA in rats. Methods: Eighteen rats were divided into three groups (n = 6): the control group, the phenylhydrazine-induced HA group and the Ech group, injected intraperitoneally with PHZ and supplemented with oral Ech daily for 6 days. Results: Ech resulted in a considerable increase in RBCs, WBCs, and platelets counts, hemoglobin, reduced glutathione, catalase, and glutathione-S-transferase levels, and a significant decrease in aspartate & alanine aminotransferases, alkaline phosphatase, gamma-glutamyl transferase, bilirubin, creatinine, urea, urate, malondialdehyde & nitric oxide levels in anemic rats. Histopathological examination of liver and kidney tissue samples showed marked improvement. Conclusion: Ech ameliorated phenylhydrazine-induced HA with a hepatorenal protective effect owing to its anti-inflammatory and antioxidant properties.

Original language	English
Article number	e16576
Journal	PeerJ
Volume	11
DOIs	https://doi.org/10.7717/peerj.16576
State	Published - 2023

Keywords

Echinochrome
Hemolytic Anemia
Oxidative stress
Phenylhydrazine

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@article{80d59cecffef47e3a6f43cdcb24277e7,

title = "Hematopoietic effect of echinochrome on phenylhydrazine-induced hemolytic anemia in rats",

abstract = "Background: Hemolytic anemia (HA) is a serious health condition resulting from reduced erythrocytes{\textquoteright} average life span. Echinochrome (Ech) is a dark-red pigment found in shells and spines of sea urchins. Aim: Studying the potential therapeutic effect of Ech on phenylhydrazine (PHZ)induced HA in rats. Methods: Eighteen rats were divided into three groups (n = 6): the control group, the phenylhydrazine-induced HA group and the Ech group, injected intraperitoneally with PHZ and supplemented with oral Ech daily for 6 days. Results: Ech resulted in a considerable increase in RBCs, WBCs, and platelets counts, hemoglobin, reduced glutathione, catalase, and glutathione-S-transferase levels, and a significant decrease in aspartate \& alanine aminotransferases, alkaline phosphatase, gamma-glutamyl transferase, bilirubin, creatinine, urea, urate, malondialdehyde \& nitric oxide levels in anemic rats. Histopathological examination of liver and kidney tissue samples showed marked improvement. Conclusion: Ech ameliorated phenylhydrazine-induced HA with a hepatorenal protective effect owing to its anti-inflammatory and antioxidant properties.",

keywords = "Echinochrome, Hemolytic Anemia, Oxidative stress, Phenylhydrazine",

author = "El-Shehry, \{Mona S.E.F.\} and Amrymi, \{Rafa A.\} and Tarek Atia and Lotfy, \{Bassant M.M.\} and Ahmed, \{Salma H.A.\} and Qutb, \{Sarah A.\} and Ali, \{Sara B.\} and Mohamed, \{Ayman S.\} and Mousa, \{Mohamed R.\} and Damanhory, \{Ahmed A.\} and Metawee, \{Mostafa E.\} and Sakr, \{Hader I.\}",

year = "2023",

doi = "10.7717/peerj.16576",

language = "English",

volume = "11",

journal = "PeerJ",

issn = "2167-8359",

publisher = "PeerJ Inc.",

}

TY - JOUR

T1 - Hematopoietic effect of echinochrome on phenylhydrazine-induced hemolytic anemia in rats

AU - El-Shehry, Mona S.E.F.

AU - Amrymi, Rafa A.

AU - Atia, Tarek

AU - Lotfy, Bassant M.M.

AU - Ahmed, Salma H.A.

AU - Qutb, Sarah A.

AU - Ali, Sara B.

AU - Mohamed, Ayman S.

AU - Mousa, Mohamed R.

AU - Damanhory, Ahmed A.

AU - Metawee, Mostafa E.

AU - Sakr, Hader I.

PY - 2023

Y1 - 2023

N2 - Background: Hemolytic anemia (HA) is a serious health condition resulting from reduced erythrocytes’ average life span. Echinochrome (Ech) is a dark-red pigment found in shells and spines of sea urchins. Aim: Studying the potential therapeutic effect of Ech on phenylhydrazine (PHZ)induced HA in rats. Methods: Eighteen rats were divided into three groups (n = 6): the control group, the phenylhydrazine-induced HA group and the Ech group, injected intraperitoneally with PHZ and supplemented with oral Ech daily for 6 days. Results: Ech resulted in a considerable increase in RBCs, WBCs, and platelets counts, hemoglobin, reduced glutathione, catalase, and glutathione-S-transferase levels, and a significant decrease in aspartate & alanine aminotransferases, alkaline phosphatase, gamma-glutamyl transferase, bilirubin, creatinine, urea, urate, malondialdehyde & nitric oxide levels in anemic rats. Histopathological examination of liver and kidney tissue samples showed marked improvement. Conclusion: Ech ameliorated phenylhydrazine-induced HA with a hepatorenal protective effect owing to its anti-inflammatory and antioxidant properties.

AB - Background: Hemolytic anemia (HA) is a serious health condition resulting from reduced erythrocytes’ average life span. Echinochrome (Ech) is a dark-red pigment found in shells and spines of sea urchins. Aim: Studying the potential therapeutic effect of Ech on phenylhydrazine (PHZ)induced HA in rats. Methods: Eighteen rats were divided into three groups (n = 6): the control group, the phenylhydrazine-induced HA group and the Ech group, injected intraperitoneally with PHZ and supplemented with oral Ech daily for 6 days. Results: Ech resulted in a considerable increase in RBCs, WBCs, and platelets counts, hemoglobin, reduced glutathione, catalase, and glutathione-S-transferase levels, and a significant decrease in aspartate & alanine aminotransferases, alkaline phosphatase, gamma-glutamyl transferase, bilirubin, creatinine, urea, urate, malondialdehyde & nitric oxide levels in anemic rats. Histopathological examination of liver and kidney tissue samples showed marked improvement. Conclusion: Ech ameliorated phenylhydrazine-induced HA with a hepatorenal protective effect owing to its anti-inflammatory and antioxidant properties.

KW - Echinochrome

KW - Hemolytic Anemia

KW - Oxidative stress

KW - Phenylhydrazine

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U2 - 10.7717/peerj.16576

DO - 10.7717/peerj.16576

M3 - Article

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SN - 2167-8359

VL - 11

JO - PeerJ

JF - PeerJ

M1 - e16576

ER -

Hematopoietic effect of echinochrome on phenylhydrazine-induced hemolytic anemia in rats

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