TY - JOUR
T1 - Effect of High Fat Diet Induced Metabolic Syndrome and Treatment with Insulin like Growth Factor 1 on Biochemical and Immunohistochemical Parameters Related to Neuronal Functions in Adult Male Albino Rats
AU - Yousuf, Asmaa Fathy
AU - Elshora, Shimaa Zakaria
AU - Abdallah, Mohamed Gaber
AU - Elmongy, Noura F.
AU - Aboelsoud, Heba Abdelnaser
AU - Mosallam, Hala Hamed
AU - Algendy, Ashraf Mohamed Mohamed
AU - El-Lateef, Abd El Lateef Saeed Abd
AU - El-Sharkawy, Abdou Mohammed
AU - Seliem, Nora
AU - El shahat, Rehab Mohamed
AU - Alkot, Ahmad Mohammad Farag
N1 - Publisher Copyright:
© 2023 National Information and Documentation Center.
PY - 2023/12
Y1 - 2023/12
N2 - The prevalence of metabolic syndrome (MetS) makes type 2 diabetes mellitus and its consequences more common in communities. Dysfunctions of the nervous system has negative implications on the lives of affected individuals and their families. This study aimed to elucidate the possible role of MetS and treatment with in insulin like growth factor 1 (IGF-1) on some biochemical and immunohistochemical parameters related to neuronal functions in adult male albino rats. For this purpose, fifty adult male albino rats were recruited and divided into five groups. (1) control group, (2) MetS group (induced by high fat diet for eight weeks), (3) MetS group treated with IGF-1 for six weeks started two weeks before induction of MetS, (4) MetS group treated with IGF-1 for six weeks started with induction of MetS, (5) MetS group treated with IGF-1 for six weeks started two weeks after starting induction of MetS. Fasting blood glucose (FBG), insulin, lipid profile, glucagon like peptide 1 (GLP1), tumor necrosis factor-α (TNF-α) and adiponectin levels were measured in the serum whereas malonaldehyde, glutathione peroxidase (GPx), brain derived neurotrophic factor (BDNF), synapsin 1 and sortilin were measured in brain tissue homogenate. Histo pathological and immunohistochemical studies for B cell lymphoma 2 (BCL2) and glial fibrillary acidic protein (GFAP) were also done. The results showed that induction of MetS caused hyperglycemia, hyperinsulinemia, dyslipidemia, elevation of the oxidant marker MDA and the proinflamatory TNF-α whereas the antioxidant GPx and the anti-infalimatory adiponectin were elevated. BDNF and the anti-apoptotic BCL2 were reduced whereas synapsin 1, sortilin and the astrocytes proliferation marker GFAP were increased. Treatment with IGF-1 was associated with improvement in all these parameters. It could be concluded that MetS is associated with reduction in neuronal protective factors and enhancement of factors which impair neuronal functions and structures. These effects may underlie the pathogenesis of cognitive dysfunction associating MetS. By antagonizing such effects of MetS, IGF-1 may be helpful in managing MetS and associated cognitive dysfunction.
AB - The prevalence of metabolic syndrome (MetS) makes type 2 diabetes mellitus and its consequences more common in communities. Dysfunctions of the nervous system has negative implications on the lives of affected individuals and their families. This study aimed to elucidate the possible role of MetS and treatment with in insulin like growth factor 1 (IGF-1) on some biochemical and immunohistochemical parameters related to neuronal functions in adult male albino rats. For this purpose, fifty adult male albino rats were recruited and divided into five groups. (1) control group, (2) MetS group (induced by high fat diet for eight weeks), (3) MetS group treated with IGF-1 for six weeks started two weeks before induction of MetS, (4) MetS group treated with IGF-1 for six weeks started with induction of MetS, (5) MetS group treated with IGF-1 for six weeks started two weeks after starting induction of MetS. Fasting blood glucose (FBG), insulin, lipid profile, glucagon like peptide 1 (GLP1), tumor necrosis factor-α (TNF-α) and adiponectin levels were measured in the serum whereas malonaldehyde, glutathione peroxidase (GPx), brain derived neurotrophic factor (BDNF), synapsin 1 and sortilin were measured in brain tissue homogenate. Histo pathological and immunohistochemical studies for B cell lymphoma 2 (BCL2) and glial fibrillary acidic protein (GFAP) were also done. The results showed that induction of MetS caused hyperglycemia, hyperinsulinemia, dyslipidemia, elevation of the oxidant marker MDA and the proinflamatory TNF-α whereas the antioxidant GPx and the anti-infalimatory adiponectin were elevated. BDNF and the anti-apoptotic BCL2 were reduced whereas synapsin 1, sortilin and the astrocytes proliferation marker GFAP were increased. Treatment with IGF-1 was associated with improvement in all these parameters. It could be concluded that MetS is associated with reduction in neuronal protective factors and enhancement of factors which impair neuronal functions and structures. These effects may underlie the pathogenesis of cognitive dysfunction associating MetS. By antagonizing such effects of MetS, IGF-1 may be helpful in managing MetS and associated cognitive dysfunction.
KW - Brain derived neurotrophic factor
KW - Cognitive dysfunction
KW - Insulin like growth factor 1
KW - Metabolic syndrome
KW - Sortilin
KW - synapsin 1
UR - http://www.scopus.com/inward/record.url?scp=85174504407&partnerID=8YFLogxK
U2 - 10.21608/EJCHEM.2023.191284.7550
DO - 10.21608/EJCHEM.2023.191284.7550
M3 - Article
AN - SCOPUS:85174504407
SN - 0449-2285
VL - 66
SP - 389
EP - 400
JO - Egyptian Journal of Chemistry
JF - Egyptian Journal of Chemistry
IS - 13
ER -
