TY - JOUR
T1 - Effect of ascorbic acid on differentiation, secretome and stemness of stem cells from human exfoliated deciduous tooth (Sheds)
AU - Bhandi, Shilpa
AU - Alkahtani, Ahmed
AU - Mashyakhy, Mohammed
AU - Abumelha, Abdulaziz S.
AU - Albar, Nassreen Hassan Mohammad
AU - Renugalakshmi, Apathsakayan
AU - Alkahtany, Mazen F.
AU - Robaian, Ali
AU - Almeslet, Asma Saleh
AU - Patil, Vikrant R.
AU - Varadarajan, Saranya
AU - Balaji, Thodur Madapusi
AU - Reda, Rodolfo
AU - Testarelli, Luca
AU - Patil, Shankargouda
N1 - Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/7
Y1 - 2021/7
N2 - Stem cells from human exfoliated deciduous teeth (SHEDs) are considered a type of mesenchymal stem cells (MSCs) because of their unique origin from the neural crest. SHEDs can self-renewal and multi-lineage differentiation with the ability to differentiate into odontoblasts, osteoblast, chondrocytes, neuronal cells, hepatocytes, adipocytes, etc. They are emerging as an ideal source of MSCs because of their easy availability and extraordinary cell number. Ascorbic acid, or vitamin C, has many cell-based applications, such as bone regeneration, osteoblastic differentiation, or extracellular matrix production. It also impacts stem cell plasticity and the ability to sustain pluripotent activity. In this study, we evaluate the effects of ascorbic acid on stemness, paracrine secretion, and differentiation into osteoblast, chondrocytes, and adipocytes. SHEDs displayed enhanced multifaceted activity, which may have applications in regenerative therapy.
AB - Stem cells from human exfoliated deciduous teeth (SHEDs) are considered a type of mesenchymal stem cells (MSCs) because of their unique origin from the neural crest. SHEDs can self-renewal and multi-lineage differentiation with the ability to differentiate into odontoblasts, osteoblast, chondrocytes, neuronal cells, hepatocytes, adipocytes, etc. They are emerging as an ideal source of MSCs because of their easy availability and extraordinary cell number. Ascorbic acid, or vitamin C, has many cell-based applications, such as bone regeneration, osteoblastic differentiation, or extracellular matrix production. It also impacts stem cell plasticity and the ability to sustain pluripotent activity. In this study, we evaluate the effects of ascorbic acid on stemness, paracrine secretion, and differentiation into osteoblast, chondrocytes, and adipocytes. SHEDs displayed enhanced multifaceted activity, which may have applications in regenerative therapy.
KW - Ascorbic acid
KW - Cytokines
KW - Differentiation
KW - Growth factors
KW - Mesenchymal stem cells
KW - SHEDs
KW - Stemness
UR - http://www.scopus.com/inward/record.url?scp=85118558089&partnerID=8YFLogxK
U2 - 10.3390/jpm11070589
DO - 10.3390/jpm11070589
M3 - Article
AN - SCOPUS:85118558089
SN - 2075-4426
VL - 11
JO - Journal of Personalized Medicine
JF - Journal of Personalized Medicine
IS - 7
M1 - 589
ER -