Dispersive MSPD Extraction Method Coupled with HPLC for the Quantification of Vitexin in Passiflora foetida Formulations

SUMMARY. A fast, simple, and efficient matrix solid phase dispersion (MSPD) coupled HPLC-UV was developed and validated for the quantification of vitexin in Passiflora foetida commercial herbal formulations. The MSPD extraction required only small quantities of sample and organic solvent for the analysis. The vi-texin was extracted from the formulations using florosil as sorbent and 80% methanol as elutant. C₁₈ HPLC column was employed for the separation, using methanol and water, in the ratio of 20:80 v/v as the mobile phase. The column was kept at 23 ± 2 °C and the flow rate was 1 mL/min. The drug detection was performed at 340 nm. The recoveries by MSPD methods were found within the range from 97.4-101.6%. This method is very effective for extracting and quantifying of vitexin in Passiflora foetida formulations with high sensitivity, precision and accuracy.