Development and Characterization of Osimertinib Loaded Ethylcellulose Based Sustained Release Nanosponges: Cytotoxicity and Biocompatibility Studies

Osimeritininb loaded nanosponges were prepared by ultrasonication technique using ethylcel-lulose and polyvinyl alcohol. Nanosponges (OS1-OS6) exhibited particle size, PDI, and zeta potential, within the range of (309.4±1.23 to 535.1±2.15 nm), (0.215±0.057 to 0.616±0.081) and (-3.11±0.036 to-4.44±0.057 mV). In contrast, entrapment efficiency and drug loading were found in the range of (51.45±2.12% to 83.36±2.21%) and (6.14±1.43%-16.17±2.12%), respectively. FTIR, DSC, XRD, and SEM studies of opti-mized OS4 showed that osimertinib was physically entangled in an amorphous state within the porous state of the nanosponge. OS4 demonstrated 92.14 ±1.01% drug release in a sustained manner for 48 h. The results of cytotoxicity and haemolytic studies revealed OS4 as a potential anticancer and biocompatible nanocarrier. Moreover, the stability study indicated OS4 has relatively similar drug release at different accelerated tem-perature conditions. In conclusion, osimertinib loaded nanosponges (OS4) could be efficient for the effective treatment of non-small cell lung cancer.