Corrigendum to “Electric injury-induced Purkinje cell apoptosis in rat cerebellum: Histological and immunohistochemical stud” [J. Chem. Neuroanat. (2017) 81 (87–96)] (S0891061816302393) (10.1016/j.jchemneu.2017.02.010)

The authors would like to apologize for any inconvenience caused. Fig. 1 Effect of electrocution on rat cerebellar Purkinje cells using H&E stain: (A) Control: flask- shaped Purkinje cells (→) with basophilic cytoplasm, vesicular, centrally located nuclei and prominent nucleoli arranged in one row between molecular (M) and granular layer (G). (B) Group 1: Deformed shrunken Purkinje cells with deeply stained cytoplasm (→) in-between normally appeared ones (►). (C) Group 2: irregular distorted and shrunken Purkinje cells with pericellular unstained halos (→). (D) Group 3: abnormal arrangement with irregular shrunken cells and pericellular unstained halos (→). (E) Group 4: fallen off cells leaving empty spaces (►), disturbed linear organization, marked cell degeneration with deeply stained cytoplasm and irregular shrunken outlines of the perikarya (→) (H&E ×1000). Fig. 2 Number of Purkinje cells in H&E stained sections in 10 non-overlapping fields from 10 different sections of each experimental group using power 200 expressed as mean SEM. a (group 1 included 10 rats that were exposed to electric current wasn't of lethal intensity for one minute, then were sacrificed immediately), b (group 2 included 10 rats that were exposed to electric current wasn't of lethal intensity for one minute, then were sacrificed after 2 h), c (group 3 included 10 rats that were exposed to electric current wasn't of lethal intensity for one minute, then were sacrificed after 4 h), d (group 4: fatal electrocution group included 10 rats that were sacrificed after being electrified up to death (153 ± 27 s). P < 0.005 significance relative to control. Fig. 3 Effect of electrocution on rat cerebellar Purkinje cells using silver stain: (A) Control: regular homogenous cytoskeletal elements in the cytoplasm of Purkinje cell perikarya (→). (B) Group 1: some Purkinje cells with mild accumulation of cytoskeletal elements (→). (C) Group 2: Purkinje cell with wide spread cytoplasmic argyrophilia (→). (D) Group 3: marked cytoplasmic argyrophilia (→). (E) Group 4: arrangement of Purkinje cells in two rows with massive accumulation of cytoskeletal elements in its perikarya (→) (Silver stain ×1000). Fig. 4 Effect of electrocution on rat cerebellar Purkinje cells as seen by electron microscope: (A) Control: (A1) large Purkinje cell (→) with euchromatic nucleus (N) and prominent nucleolus (×1000). A2) a higher magnification of the previous image showing nucleus (N), ribosomes (→), RER (►), Golgi apparatus (G) and numerous electron dense mitochondria (M) (x 3000). (B) Group 1: (B1) decreased cell size with surface irregularity (→) (×1000). (B2) a higher magnification of the previous image showing perinuclear space (→), slightly dilated RER (►) and Golgi apparatus (G) and secondary lysosomes (two arrows) (×3000). (C) Group 2: (C1) irregular shrunken Purkinje cell (→) (×1000). (C2) a higher magnification of the previous image showing nuclear irregularity (N), dilated RER (→) and Golgi apparatus (G), swollen degenerated mitochondria (M) and secondary lysosomes (►) (×3000). (D) Group 3: (D1) Dark shrunken Purkinje cell in comparison to the control with irregular surface (→) (×1000). (D2) a higher magnification of the previous image showing nucleus with disturbed outlines (N), dilated RER (→) and Golgi apparatus (►), swollen mitochondria with destructed cristae (M) (×3000). (E) Group 4: (E1) severely contracted dark stained cell with irregular outlines and spinous processes on its surface (→) (×1000). (E2) a higher magnification of the previous image showing nuclear irregularities with nuclear indentation (N), markedly dilated RER (→) and Golgi apparatus (G), markedly swollen degenerated mitochondria with destructed cristae (M) (×3000). Fig. 5 Effect of electrocution on rat cerebellar Purkinje cells using capase-3 immunohistochemistry: (A) Control: negative immune reaction of most of Purkinje cells (►) except for scattered cells that exhibited faint light brown granules in their cytoplasm (→). (B) Group 1: few caspase-3 positive cells (→). (C) Group 2: moderate reaction of many of Purkinje cells (→). (D) Group 3: moderate reaction of most of Purkinje cells (→). (E) Group 4: marked positive caspase-3 reaction (→) (Caspase–3 1000).