Chromium resistant microbes and melatonin reduced Cr uptake and toxicity, improved physio-biochemical traits and yield of wheat in contaminated soil

Melatonin and metal resistant microbes can enhance plant defense responses against various abiotic stresses, but little is known about the combined effects of melatonin and chromium (Cr) resistant microbes on reducing Cr toxicity in wheat (Triticum aestivum L.). In current study, we examined the effects of combined application of melatonin (0, 1, 2 mM) and Bacillus subtilis (with and without inoculation) on wheat physio-biochemical responses and Cr uptake under different levels of Cr (0, 25, 50 and 100 mg Cr kg⁻¹ DM soil). Chromium stress decreased the wheat growth, biomass, chlorophyll and relative water contents by causing oxidative damage in the form of overproduction of electrolyte leakage, hydrogen peroxide and malondialdehyde. However, foliar application of melatonin enhanced the plant growth, biomass and photosynthesis by alleviating the oxidative damage and Cr accumulation by plants. Melatonin significantly increased the enzymatic and non-enzymatic antioxidant activities as compared with respective control. Inoculation with microbes further enhanced the positive impacts of melatonin on wheat growth and reduced the Cr uptake by plants. Compared with non-inoculation and melatonin treatment, the inoculation with B. subtilis increased cholorophyll a by 27%, cholorophyll b by 49%, ascorbic acid in leaves by 50% and soluble proteins by 72% in wheat grwon with 50 mg Cr kg⁻¹ DM soil. The application of B. subtilis reduced oxidative stress and Cr toxicity by transforming the Cr⁶⁺ to Cr³⁺ in shoots and roots of wheat. Furthermore, B. subtilis reduced the Cr⁶⁺ uptake by wheat plants. The result of the present study revealed that the combined application of melatonin and B. subtilis might be a feasible approach aiming to reduce the Cr toxicity and its accumulation by wheat and probably in other plants.