TY - JOUR
T1 - Antitumor activity of extract and isolated compounds from Drechslera rostrata and Eurotium tonophilum
AU - Alasmary, Fatmah A.S.
AU - Awaad, Amani S.
AU - Kamal, Mehnaz
AU - Alqasoumi, Saleh I.
AU - Zain, Mohamed E.
N1 - Publisher Copyright:
© 2017 The Authors
PY - 2018/2
Y1 - 2018/2
N2 - Total extracts of Drechslera rostrata and Eurotium tonophilum in addition of two isolated compounds from their cultures [di-2-ethylhexyl phthalate (H1) and 1,8-Dihydroxy-3-methoxy-6-methyl-anthraquinone (H2)] were tested for their antitumor activity using four human carcinoma cell lines. Antitumor activity was assessed by performing MTT assay to check the % cell viability. The % viability of HCT-116 (colon carcinoma), HeLa (cervical carcinoma), HEp-2 (larynx carcinoma) and HepG-2 (hepatocellular carcinoma) cells decreased after treatment with Drechslera rostrata and Eurotium tonophilum extracts, these effects were ranged from 059.0 ± 0.1 to 217.0 ± 0.3 µg/ml on all types of cancer cells. The best activity was recorded for Eurotium tonpholium extract (054.5 ± 0.3, 059.0 ± 0.5 and 059.0 ± 0.1 for HEp-2, Hela, and HepG-2 respectively). The isolated compounds (H1&H2) were found to be responsible about the activities because they recorded the lowest IC50 on tested cell lines with range of 9.5–20.3 μg/ml. Vinblastine sulphate was used as a reference standard and showed in vitro anticancer activity. This study demonstrated that all extracts and isolated compounds have antitumor activity against HCT-116, HeLa, HEp-2 and HepG-2 cells.
AB - Total extracts of Drechslera rostrata and Eurotium tonophilum in addition of two isolated compounds from their cultures [di-2-ethylhexyl phthalate (H1) and 1,8-Dihydroxy-3-methoxy-6-methyl-anthraquinone (H2)] were tested for their antitumor activity using four human carcinoma cell lines. Antitumor activity was assessed by performing MTT assay to check the % cell viability. The % viability of HCT-116 (colon carcinoma), HeLa (cervical carcinoma), HEp-2 (larynx carcinoma) and HepG-2 (hepatocellular carcinoma) cells decreased after treatment with Drechslera rostrata and Eurotium tonophilum extracts, these effects were ranged from 059.0 ± 0.1 to 217.0 ± 0.3 µg/ml on all types of cancer cells. The best activity was recorded for Eurotium tonpholium extract (054.5 ± 0.3, 059.0 ± 0.5 and 059.0 ± 0.1 for HEp-2, Hela, and HepG-2 respectively). The isolated compounds (H1&H2) were found to be responsible about the activities because they recorded the lowest IC50 on tested cell lines with range of 9.5–20.3 μg/ml. Vinblastine sulphate was used as a reference standard and showed in vitro anticancer activity. This study demonstrated that all extracts and isolated compounds have antitumor activity against HCT-116, HeLa, HEp-2 and HepG-2 cells.
KW - 1,8-Dihydroxy-3-methoxy-6-methyl-anthraquinone
KW - Di-2-ethylhexyl phthalate
KW - Drechslera rostrata
KW - Eurotium tonophilum
KW - Human cancer cell lines
KW - MTT assay
UR - http://www.scopus.com/inward/record.url?scp=85036495269&partnerID=8YFLogxK
U2 - 10.1016/j.jsps.2017.11.011
DO - 10.1016/j.jsps.2017.11.011
M3 - Article
AN - SCOPUS:85036495269
SN - 1319-0164
VL - 26
SP - 279
EP - 285
JO - Saudi Pharmaceutical Journal
JF - Saudi Pharmaceutical Journal
IS - 2
ER -
