ACUTE AND SUB-ACUTE ORAL TOXICITY OF PTEROSTILBENE, DOCOSAHEXAENOIC ACID AND ITS COMBINATION IN SPRAGUE DAWLEY RATS

The toxic effects of acute and sub-acute administrations of Pterostilbene (PTR), Docosahexaenoic acid (DHA), and Pterostilbene + Docosahexaenoic acid combinations (PTR+DHA) were studied in rats. In acute oral toxicity, the effects of a single oral dose of PTR (2000 mg/kg), DHA (2000 mg/kg), and PTR (2000 mg/kg) + DHA (2000 mg/kg) combinations have been evaluated. Therein animal behavior and mortality were observed for 14 days. In the sub-acute study, the PTR (200 mg/kg), DHA (200 mg/kg) and PTR+DHA (200 mg/kg + 200 mg/kg) combinations were administered ones a day for 28 days. The body weight, organ weight, food, water intake, biochemical, hematological parameters and histopathology of the liver were studied. No mortality or morbidity was observed during acute and sub-acute toxicity studies in rats. Additionally, no significant variations were observed in relative organ weight, hematological and biochemical parameters in treated groups as compared to the control group. Moreover, there were no noticeable histological changes observed in the liver of treated groups as compared to the control group. The oral administration of PTR, DHA and (PTR+DHA) combinations did not produce any significant toxic effect in rats. No toxic effects were observed during acute and sub-acute toxicity studies. Overall PTR, DHA and (PTR+DHA) combinations are safe natural bio-actives as studied. Further cytotoxicity and genotoxicity studies of the above drug(s) or their combinations may be executed for appreciative safety.