TY - JOUR
T1 - Acaciasaligna extract alleviates quetiapine-induced sexual toxicity in male albino rats
T2 - Insights from UPLC-MS/MS metabolite profiling, structural and PI3K/NF-κB pathway assessments
AU - Yousof, Shimaa Mohammad
AU - Shehata, Shaimaa A.
AU - Ismail, Ezzat A.
AU - Abd El-moneam, Samar M.
AU - Mansour, Basma S.A.
AU - Farag, Mohamed A.
AU - Elshamy, Abdelsamed I.
AU - El-Nasser G. El Gendy, Abd
AU - Serag, Ahmed
AU - Abd El-Fadeal, Noha M.
AU - Abdel-Karim, Rehab Ibrahim
AU - Mostafa, Mostafa M.
AU - El-Sheikh, Dina H.
AU - Zayed, Mohamed A.
N1 - Publisher Copyright:
© 2024 The Authors
PY - 2024/7/15
Y1 - 2024/7/15
N2 - Background: Quetiapine (QET) abuse has increased due to its anxiolytic and hedonic effects, necessitating protective adjunct treatments. Acacia saligna (A. saligna) flowers, used in traditional medicine, have potential health benefits. Aim: To investigate the protective role of A. saligna flower extract against QET-induced sexual toxicity, and to elucidate the possible underlying mechanisms through metabolomic and physiological studies. Methods: A. saligna extract was subjected to metabolite profiling via High-Resolution Ultra-Performance Liquid Chromatography-Mass Spectrometry (UPLC-ESI–qTOF-MS). Forty-eight adult male albino rats were assigned into six groups for 30 days. The intracavernosal pressure (ICP), semen, biochemical, hormonal, histological, genetic and Western blot (WB) analyses were determined. Results: A. saligna extract is rich in phenolic compounds, flavonoids, tannins, and unsaturated fatty acids. QET significantly decreased ICP and negatively affected semen parameters. A. saligna mitigated decreased sperm motility and ameliorated overexpressed proinflammatory genes in QET-55 group. A. saligna ameliorated the reduction of the antioxidant biomarkers, testosterone, luteinizing hormone (LH), and follicle-stimulating hormone (FSH), concurrent with downregulation of the nuclear factor kappa B (NF-κB) protein. A. saligna counteracted the disrupted testicular and prostatic structures revealed by histological examination. Conclusion: The extract from A. saligna, which contains a high concentration of antioxidants and anti-inflammatory chemicals, effectively mitigates sexual toxicity caused by QET. This study provided the first known explanation of the hypothesized processes behind the protective properties of A. saligna through biological, biochemical, and histological parameters. The results emphasize the potential of A. saligna as a safeguarding agent against drug-induced sexual toxicity.
AB - Background: Quetiapine (QET) abuse has increased due to its anxiolytic and hedonic effects, necessitating protective adjunct treatments. Acacia saligna (A. saligna) flowers, used in traditional medicine, have potential health benefits. Aim: To investigate the protective role of A. saligna flower extract against QET-induced sexual toxicity, and to elucidate the possible underlying mechanisms through metabolomic and physiological studies. Methods: A. saligna extract was subjected to metabolite profiling via High-Resolution Ultra-Performance Liquid Chromatography-Mass Spectrometry (UPLC-ESI–qTOF-MS). Forty-eight adult male albino rats were assigned into six groups for 30 days. The intracavernosal pressure (ICP), semen, biochemical, hormonal, histological, genetic and Western blot (WB) analyses were determined. Results: A. saligna extract is rich in phenolic compounds, flavonoids, tannins, and unsaturated fatty acids. QET significantly decreased ICP and negatively affected semen parameters. A. saligna mitigated decreased sperm motility and ameliorated overexpressed proinflammatory genes in QET-55 group. A. saligna ameliorated the reduction of the antioxidant biomarkers, testosterone, luteinizing hormone (LH), and follicle-stimulating hormone (FSH), concurrent with downregulation of the nuclear factor kappa B (NF-κB) protein. A. saligna counteracted the disrupted testicular and prostatic structures revealed by histological examination. Conclusion: The extract from A. saligna, which contains a high concentration of antioxidants and anti-inflammatory chemicals, effectively mitigates sexual toxicity caused by QET. This study provided the first known explanation of the hypothesized processes behind the protective properties of A. saligna through biological, biochemical, and histological parameters. The results emphasize the potential of A. saligna as a safeguarding agent against drug-induced sexual toxicity.
KW - Acacia saligna
KW - Oxidative stress
KW - P13K/NF-κB pathway
KW - Quetiapine
KW - Sexual toxicity
KW - UPLC-MS/MS
UR - http://www.scopus.com/inward/record.url?scp=85198185316&partnerID=8YFLogxK
U2 - 10.1016/j.heliyon.2024.e33993
DO - 10.1016/j.heliyon.2024.e33993
M3 - Article
AN - SCOPUS:85198185316
SN - 2405-8440
VL - 10
JO - Heliyon
JF - Heliyon
IS - 13
M1 - e33993
ER -