α-Chymotrypsin regulates free fatty acids and UCHL-1 to ameliorate N-methyl nitrosourea induced mammary gland carcinoma in albino wistar rats

Asha Rani; Subhadeep Roy; Manjari Singh; Uma Devi; Rajnish K. Yadav; Swetlana Gautam; Jitendra K. Rawat; Mohd Nazam Ansari; Abdulaziz S. Saeedan; Anand Prakash; Gaurav Kaithwas

doi:10.1007/s10787-016-0280-5

α-Chymotrypsin regulates free fatty acids and UCHL-1 to ameliorate N-methyl nitrosourea induced mammary gland carcinoma in albino wistar rats

Asha Rani, Subhadeep Roy, Manjari Singh, Uma Devi, Rajnish K. Yadav, Swetlana Gautam, Jitendra K. Rawat, Mohd Nazam Ansari, Abdulaziz S. Saeedan, Anand Prakash, Gaurav Kaithwas

Pharmacology

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

This study was undertaken to investigate the effect of α-chymotrypsin on methyl nitrosourea (MNU) induced mammary gland carcinoma in albino wistar rats. Animals were randomized into four groups (six animals in each). Group I (sham control 0.9 % normal saline p.o.); Group II (toxic control, MNU 47 mg/kg, i.v.); Group III (α-chymotrypsin, 5 mg/kg, p.o.); Group IV (α-chymotrypsin, 10 mg/kg p.o.). Toxicity was induced by single i.v. injection of MNU followed by α-chymotrypsin supplementation therapy for 100 days. MNU treatment was evident with increased alveolar bud count, differentiation score, upregulated inflammatory enzymes markers (COX, LOX and NO) antioxidative stress markers (TBARs, SOD, catalase and GSH).MNU associated toxicity was also ascertained by PGP 9.5 and NF-κB expression in the mammary gland tissue followed by FAME analysis for fatty acid profiling. α-chymotrypsin afforded significant protection against the deleterious effects of MNU.

Original language	English
Pages (from-to)	277-286
Number of pages	10
Journal	Inflammopharmacology
Volume	24
Issue number	5
DOIs	https://doi.org/10.1007/s10787-016-0280-5
State	Published - 1 Oct 2016

Keywords

FAME
Mammary gland
NF-κB p65
Oxidative stress
PGP 9.5
α-Chymotrypsin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Rani, A., Roy, S., Singh, M., Devi, U., Yadav, R. K., Gautam, S., Rawat, J. K., Ansari, M. N., Saeedan, A. S., Prakash, A., & Kaithwas, G. (2016). α-Chymotrypsin regulates free fatty acids and UCHL-1 to ameliorate N-methyl nitrosourea induced mammary gland carcinoma in albino wistar rats. Inflammopharmacology, 24(5), 277-286. https://doi.org/10.1007/s10787-016-0280-5

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title = "α-Chymotrypsin regulates free fatty acids and UCHL-1 to ameliorate N-methyl nitrosourea induced mammary gland carcinoma in albino wistar rats",

abstract = "This study was undertaken to investigate the effect of α-chymotrypsin on methyl nitrosourea (MNU) induced mammary gland carcinoma in albino wistar rats. Animals were randomized into four groups (six animals in each). Group I (sham control 0.9 \% normal saline p.o.); Group II (toxic control, MNU 47 mg/kg, i.v.); Group III (α-chymotrypsin, 5 mg/kg, p.o.); Group IV (α-chymotrypsin, 10 mg/kg p.o.). Toxicity was induced by single i.v. injection of MNU followed by α-chymotrypsin supplementation therapy for 100 days. MNU treatment was evident with increased alveolar bud count, differentiation score, upregulated inflammatory enzymes markers (COX, LOX and NO) antioxidative stress markers (TBARs, SOD, catalase and GSH).MNU associated toxicity was also ascertained by PGP 9.5 and NF-κB expression in the mammary gland tissue followed by FAME analysis for fatty acid profiling. α-chymotrypsin afforded significant protection against the deleterious effects of MNU.",

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author = "Asha Rani and Subhadeep Roy and Manjari Singh and Uma Devi and Yadav, \{Rajnish K.\} and Swetlana Gautam and Rawat, \{Jitendra K.\} and Ansari, \{Mohd Nazam\} and Saeedan, \{Abdulaziz S.\} and Anand Prakash and Gaurav Kaithwas",

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doi = "10.1007/s10787-016-0280-5",

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T1 - α-Chymotrypsin regulates free fatty acids and UCHL-1 to ameliorate N-methyl nitrosourea induced mammary gland carcinoma in albino wistar rats

AU - Rani, Asha

AU - Roy, Subhadeep

AU - Singh, Manjari

AU - Devi, Uma

AU - Yadav, Rajnish K.

AU - Gautam, Swetlana

AU - Rawat, Jitendra K.

AU - Ansari, Mohd Nazam

AU - Saeedan, Abdulaziz S.

AU - Prakash, Anand

AU - Kaithwas, Gaurav

PY - 2016/10/1

Y1 - 2016/10/1

N2 - This study was undertaken to investigate the effect of α-chymotrypsin on methyl nitrosourea (MNU) induced mammary gland carcinoma in albino wistar rats. Animals were randomized into four groups (six animals in each). Group I (sham control 0.9 % normal saline p.o.); Group II (toxic control, MNU 47 mg/kg, i.v.); Group III (α-chymotrypsin, 5 mg/kg, p.o.); Group IV (α-chymotrypsin, 10 mg/kg p.o.). Toxicity was induced by single i.v. injection of MNU followed by α-chymotrypsin supplementation therapy for 100 days. MNU treatment was evident with increased alveolar bud count, differentiation score, upregulated inflammatory enzymes markers (COX, LOX and NO) antioxidative stress markers (TBARs, SOD, catalase and GSH).MNU associated toxicity was also ascertained by PGP 9.5 and NF-κB expression in the mammary gland tissue followed by FAME analysis for fatty acid profiling. α-chymotrypsin afforded significant protection against the deleterious effects of MNU.

AB - This study was undertaken to investigate the effect of α-chymotrypsin on methyl nitrosourea (MNU) induced mammary gland carcinoma in albino wistar rats. Animals were randomized into four groups (six animals in each). Group I (sham control 0.9 % normal saline p.o.); Group II (toxic control, MNU 47 mg/kg, i.v.); Group III (α-chymotrypsin, 5 mg/kg, p.o.); Group IV (α-chymotrypsin, 10 mg/kg p.o.). Toxicity was induced by single i.v. injection of MNU followed by α-chymotrypsin supplementation therapy for 100 days. MNU treatment was evident with increased alveolar bud count, differentiation score, upregulated inflammatory enzymes markers (COX, LOX and NO) antioxidative stress markers (TBARs, SOD, catalase and GSH).MNU associated toxicity was also ascertained by PGP 9.5 and NF-κB expression in the mammary gland tissue followed by FAME analysis for fatty acid profiling. α-chymotrypsin afforded significant protection against the deleterious effects of MNU.

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KW - Oxidative stress

KW - PGP 9.5

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α-Chymotrypsin regulates free fatty acids and UCHL-1 to ameliorate N-methyl nitrosourea induced mammary gland carcinoma in albino wistar rats

Abstract

Keywords

UN SDGs

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