Tracking glycosylation in live cells using FTIR spectroscopy

Joshua Phelan; Ali Altharawi; K. L.Andrew Chan

doi:10.1016/j.talanta.2020.120737

Tracking glycosylation in live cells using FTIR spectroscopy

Joshua Phelan, Ali Altharawi, K. L.Andrew Chan

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

This is the first demonstration of the study of glycan protein turnover in living cells by FTIR with commercially available tetraacetylated N-Azidoacetyl-D-Mannosamine (Ac₄ManNAz) label. The FTIR analysis has shown to be able to monitor the metabolism of glycans in living cells in real time. The method is simple, quantitative and requires equipment that are available in many laboratories. It can be used in a wide range of applications such as the study of glycosylation and cell-signalling.

Original language	English
Article number	120737
Journal	Talanta
Volume	211
DOIs	https://doi.org/10.1016/j.talanta.2020.120737
State	Published - 1 May 2020
Externally published	Yes

Keywords

Alkyne
ATR
Azide
FT-IR
Live cells
Metabolic labelling
Vibrational spectroscopy

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10.1016/j.talanta.2020.120737

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title = "Tracking glycosylation in live cells using FTIR spectroscopy",

abstract = "This is the first demonstration of the study of glycan protein turnover in living cells by FTIR with commercially available tetraacetylated N-Azidoacetyl-D-Mannosamine (Ac4ManNAz) label. The FTIR analysis has shown to be able to monitor the metabolism of glycans in living cells in real time. The method is simple, quantitative and requires equipment that are available in many laboratories. It can be used in a wide range of applications such as the study of glycosylation and cell-signalling.",

keywords = "Alkyne, ATR, Azide, FT-IR, Live cells, Metabolic labelling, Vibrational spectroscopy",

author = "Joshua Phelan and Ali Altharawi and Chan, \{K. L.Andrew\}",

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doi = "10.1016/j.talanta.2020.120737",

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AU - Altharawi, Ali

AU - Chan, K. L.Andrew

PY - 2020/5/1

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N2 - This is the first demonstration of the study of glycan protein turnover in living cells by FTIR with commercially available tetraacetylated N-Azidoacetyl-D-Mannosamine (Ac4ManNAz) label. The FTIR analysis has shown to be able to monitor the metabolism of glycans in living cells in real time. The method is simple, quantitative and requires equipment that are available in many laboratories. It can be used in a wide range of applications such as the study of glycosylation and cell-signalling.

AB - This is the first demonstration of the study of glycan protein turnover in living cells by FTIR with commercially available tetraacetylated N-Azidoacetyl-D-Mannosamine (Ac4ManNAz) label. The FTIR analysis has shown to be able to monitor the metabolism of glycans in living cells in real time. The method is simple, quantitative and requires equipment that are available in many laboratories. It can be used in a wide range of applications such as the study of glycosylation and cell-signalling.

KW - Alkyne

KW - ATR

KW - Azide

KW - FT-IR

KW - Live cells

KW - Metabolic labelling

KW - Vibrational spectroscopy

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SN - 0039-9140

VL - 211

JO - Talanta

JF - Talanta

M1 - 120737

ER -

Tracking glycosylation in live cells using FTIR spectroscopy

Abstract

Keywords

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