Laser-assisted synthesis of Mn_0.50Zn_0.50Fe₂O₄ nanomaterial: Characterization and in vitro inhibition activity towards bacillus subtilis biofilm

There is growing interest in the development of novel nanomaterials with potential antimicrobial activity and lesser toxicity. In the current research work, Mn_0.5Zn_0.5Fe₂O₄ nanoparticles were synthesized via a novel coprecipitation cum laser ablation technique yielding fine spinal structured material. The synthesized nanomaterial was structurally characterized by X-ray diffraction technique which confirmed the formation and the crystalline nature of Mn_0.50Zn_0.50Fe₂O₄ nanomaterial. The crystallite size determined by Debye-Scherrer's formula was found to be 12 nm. The formation of nanoparticles was evidenced by scanning electron microscopy. Energy dispersive X-ray analysis (EDXA) was performed for elemental analysis. The synthesized nanomaterial was interestingly found to be an effective antimicrobial agent and inhibited the growth of Bacillus subtilis biofilm formation. The 5 μg of Mn_0.5Zn_0.5Fe₂O₄ nanomaterial dissolved in 1 mL of DMSO showed excellent biofilm inhibitory activity 91.23% ± 1.87 against Bacillus subtilis.