Investigating antibody-antigen binding with atomic force microscopy

O. Ouerghi; A. Touhami; A. Othmane; H. Ben Ouada; C. Martelet; C. Fretigny; N. Jaffrezic-Renault

doi:10.1016/S0925-4005(02)00020-5

Investigating antibody-antigen binding with atomic force microscopy

O. Ouerghi
, A. Touhami
, A. Othmane
, H. Ben Ouada
, C. Martelet
, C. Fretigny
, N. Jaffrezic-Renault

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

Adhesive interactions between antigen-antibody play a central role in immune system operation. The objective of this paper is to detect immune complexes without any label, by using atomic force microscopy (AFM). We have proceeded by imaging antibody (anti-rabbit IgG) or anti-rabbit IgG moieties adsorbed onto mica surface, before and after incubation of two kinds of antigens: a specific (rabbit IgG) and a non-pecific one (sheep IgG). The analysis using the height histograms reveals many interesting features. We propose a general framework for interpreting height histogram measurements, which enable the discrimination between specific and non-specific complexes.

Original language	English
Pages (from-to)	167-175
Number of pages	9
Journal	Sensors and Actuators B: Chemical
Volume	84
Issue number	2-3
DOIs	https://doi.org/10.1016/S0925-4005(02)00020-5
State	Published - 15 May 2002
Externally published	Yes

Keywords

Atomic force microscopy
Biosensors
IgG
IgG moieties
Immune complex

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10.1016/S0925-4005(02)00020-5

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title = "Investigating antibody-antigen binding with atomic force microscopy",

abstract = "Adhesive interactions between antigen-antibody play a central role in immune system operation. The objective of this paper is to detect immune complexes without any label, by using atomic force microscopy (AFM). We have proceeded by imaging antibody (anti-rabbit IgG) or anti-rabbit IgG moieties adsorbed onto mica surface, before and after incubation of two kinds of antigens: a specific (rabbit IgG) and a non-pecific one (sheep IgG). The analysis using the height histograms reveals many interesting features. We propose a general framework for interpreting height histogram measurements, which enable the discrimination between specific and non-specific complexes.",

keywords = "Atomic force microscopy, Biosensors, IgG, IgG moieties, Immune complex",

author = "O. Ouerghi and A. Touhami and A. Othmane and \{Ben Ouada\}, H. and C. Martelet and C. Fretigny and N. Jaffrezic-Renault",

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T1 - Investigating antibody-antigen binding with atomic force microscopy

AU - Ouerghi, O.

AU - Touhami, A.

AU - Othmane, A.

AU - Ben Ouada, H.

AU - Martelet, C.

AU - Fretigny, C.

AU - Jaffrezic-Renault, N.

PY - 2002/5/15

Y1 - 2002/5/15

N2 - Adhesive interactions between antigen-antibody play a central role in immune system operation. The objective of this paper is to detect immune complexes without any label, by using atomic force microscopy (AFM). We have proceeded by imaging antibody (anti-rabbit IgG) or anti-rabbit IgG moieties adsorbed onto mica surface, before and after incubation of two kinds of antigens: a specific (rabbit IgG) and a non-pecific one (sheep IgG). The analysis using the height histograms reveals many interesting features. We propose a general framework for interpreting height histogram measurements, which enable the discrimination between specific and non-specific complexes.

AB - Adhesive interactions between antigen-antibody play a central role in immune system operation. The objective of this paper is to detect immune complexes without any label, by using atomic force microscopy (AFM). We have proceeded by imaging antibody (anti-rabbit IgG) or anti-rabbit IgG moieties adsorbed onto mica surface, before and after incubation of two kinds of antigens: a specific (rabbit IgG) and a non-pecific one (sheep IgG). The analysis using the height histograms reveals many interesting features. We propose a general framework for interpreting height histogram measurements, which enable the discrimination between specific and non-specific complexes.

KW - Atomic force microscopy

KW - Biosensors

KW - IgG

KW - IgG moieties

KW - Immune complex

UR - https://www.scopus.com/pages/publications/0037095341

U2 - 10.1016/S0925-4005(02)00020-5

DO - 10.1016/S0925-4005(02)00020-5

M3 - Article

AN - SCOPUS:0037095341

SN - 0925-4005

VL - 84

SP - 167

EP - 175

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

IS - 2-3

ER -

Investigating antibody-antigen binding with atomic force microscopy

Abstract

Keywords

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