Hydrothermal assisted α-amylase-modified silver nanoparticles (α-Amy-AgNPs): Multi-functional nanocatalyst for dye degradation and biomedical applications

This study reports the eco-friendly synthesis of silver nanoparticles (α-Amy-AgNPs) using α-amylase derived from Avena fatua seeds, which functioned as a bioreductant and stabilizing agent. The α-Amy-AgNPs exhibited well-defined crystalline structure, nanoscale size, and stable spherical morphology, as confirmed by spectroscopic and microscopic analyses. Significantly, α-Amy-AgNPs exhibited strong multifunctional capabilities, fully degrading methylene blue (MB) under visible light and maintaining high catalytic activity even after repeated use. They also demonstrated effective antibacterial properties against multidrug-resistant pathogens, along with notable antioxidant activity. Additionally, cytotoxicity assessment using Artemia salina indicated moderate biological activity with dose-dependent safety, highlighting their suitability for biomedical applications. These findings underscore the potential of α-amylase-mediated AgNPs as sustainable nanomaterials for environmental remediation and therapeutic development.