Abstract
A high-performance thin layer chromatographic densitometric method for the analysis of arbutin was developed and validated in the present investigation. Arbutin was separated on aluminium-backed silica gel 60 F 254 plates with methanol : chloroform (3 : 7)% (v/v) as the mobile phase. This system was found to give a compact spot of arbutin at a retention factor (R f) value of 0.32±0.02. The limit of detection and limit of quantification were found to be 35.42 and 106.26 ng/spot, respectively. The proposed method with a high degree of precision and accuracy was employed for the analysis of arbutin in the bulk drug and methanolic extract of Arctostaphylos uva-ursi.
| Original language | English |
|---|---|
| Pages (from-to) | 1671-1675 |
| Number of pages | 5 |
| Journal | Natural Product Research |
| Volume | 25 |
| Issue number | 17 |
| DOIs | |
| State | Published - Oct 2011 |
Keywords
- Arbutin
- Arctostaphylos uva-ursi
- HPTLC densitometry
- ICH guidelines