TY - JOUR
T1 - Green high-performance thin-layer chromatography (HPTLC) approaches for pharmaceutical and drug analysis
AU - Fiza, Ashna
AU - Shakeel, Faiyaz
AU - Khan, Alina
AU - Alqarni, Mohammed H.
AU - Foudah, Ahmed I.
AU - Alam Mohammad, Prawez
N1 - Publisher Copyright:
© 2025 Elsevier B.V.
PY - 2026/2
Y1 - 2026/2
N2 - Conventional liquid chromatography, though widely used in drug discovery, quality control, and stability studies, raises concerns regarding solvent consumption, analyst safety, and environmental impact. Recently, green analytical chemistry has guided the adoption of eco-friendly techniques, among which high-performance thin-layer chromatography (HPTLC) stands out for its versatility, low solvent requirements, parallel analysis capabilities, and robustness. This review highlights recent advancements in green HPTLC methodologies, emphasizing the use of sustainable solvents, validation metrics, and greenness tools. Special focus is given to applications across single and multi-analytes determination, illustrating the technique's growing relevance in pharmacokinetic, pharmacodynamic, and bioequivalence studies. Comparative analysis of greenness scores demonstrate the potential of green HPTLC to achieve high analytical performance while minimizing environmental burden. Future perspectives include enhanced miniaturization, automation, integration with hyphenated techniques, and regulatory harmonization. This review underscores green HPTLC as a pivotal approach for sustainable pharmaceutical analysis, bridging analytical efficiency with environmental stewardship.
AB - Conventional liquid chromatography, though widely used in drug discovery, quality control, and stability studies, raises concerns regarding solvent consumption, analyst safety, and environmental impact. Recently, green analytical chemistry has guided the adoption of eco-friendly techniques, among which high-performance thin-layer chromatography (HPTLC) stands out for its versatility, low solvent requirements, parallel analysis capabilities, and robustness. This review highlights recent advancements in green HPTLC methodologies, emphasizing the use of sustainable solvents, validation metrics, and greenness tools. Special focus is given to applications across single and multi-analytes determination, illustrating the technique's growing relevance in pharmacokinetic, pharmacodynamic, and bioequivalence studies. Comparative analysis of greenness scores demonstrate the potential of green HPTLC to achieve high analytical performance while minimizing environmental burden. Future perspectives include enhanced miniaturization, automation, integration with hyphenated techniques, and regulatory harmonization. This review underscores green HPTLC as a pivotal approach for sustainable pharmaceutical analysis, bridging analytical efficiency with environmental stewardship.
KW - Drug formulation
KW - Green analytical chemistry
KW - Greenness metric
KW - HPTL
KW - Sample preparation
KW - Validation
UR - https://www.scopus.com/pages/publications/105025102417
U2 - 10.1016/j.trac.2025.118606
DO - 10.1016/j.trac.2025.118606
M3 - Review article
AN - SCOPUS:105025102417
SN - 0165-9936
VL - 195
JO - TrAC - Trends in Analytical Chemistry
JF - TrAC - Trends in Analytical Chemistry
M1 - 118606
ER -