GC-MS/MS Based Metabolite Profiling and Evaluation of Antimicrobial Properties of Emblica officinalis Leaves Extract.

Ever since, traditional plants and their products were consumed by human beings in diverse health matters to heal variety of infections. At the moment, in all over globe, the scientific attention and focuses are escalating with regard to phytomedicine. This study explores the value of secondary metabolites of Emblica officinalis leaves extract (EOLE) identified through GC-MS/MS profiling in different solvents against microbial strains. In-vitro antimicrobial activity was established by means of an agar well diffusion scheme at various loaded concentrations (25 μg/mL-1000 μg/mL). GC-MS/MS profiling confirmed the presence of major accredited compounds characterized as PDMTFM (Rt- 9.891 min and peak area-1.77%), P2,6-BDME (Rt-8.588 min, peak area - 100%), P2,5-BDME (Rt - 8.567 min, peak area - 36.78%) and BFTHTM (Rt-9.055 min, peak area - 7.87%) in tag plant. Gram positive bacteria; Bacillus pumilis, Bacillus cereus, Staphylococcus pyogenous, Bacillus polymyxa and Gram negative bacteria; Vibrio cholera, Providencia alcalifaciens, while fungal species; Neurospora crassa, Trichoderma viridae, Aspergillus brasileinsis and Cladosporium oxysporum were used during this study. Aqueous extract made known excellent antimicrobial activity than other selected extract. Inhibitory values of aqueous and methanolic extract were measured with reference to ciprofloxacin and amphotericin. Preliminary results of this study implied that EOLE could be considered as strong antimicrobial agent to treat human infectious diseases.