Designing and investigation of a novel packaging film based on CTAB-encapsulated azoloazine derivatives and carboxymethyl cellulose

The target of this article is to create and characterize a novel antimicrobial packaging film based on a highly efficient antimicrobial azoloazine derivative (H13: 10-((E)-2-chlorobenzylidene)-2-((E)-4-chlorobenzylidene)-5-(2-chlorophenyl)-5,6,7,8,9,10 hexahydrocyclohepta[d] thiazolo [3,2-a]pyrimidin-3(2H)-one). Different H13 contents (50, 100, and 150 µg) was encapsulated into cetyltrimethylammonium bromide surfactant micelles (CTAB). The anionic carboxymethyl cellulose (CMC) was assembled by positive-charge CTAB micelles to make a novel antimicrobial packaging film (H13@CTAB/CMC). H13 structure was analysed by ¹HNMR. The cytotoxicity test of H13 was analyzed. The maximum cytotoxicity was 66.92 % at concentration (150 µg). H13@CTAB/CMC film was explored using ATR-IR, XRD, XPS, thermal analysis, and antimicrobial inhibition experiments. The SEM, water vapor permeability, water vapor sorption isotherms, mechanical, and thermal properties were studied. The H13 (150)@CTAB/CMC had the lowest WVP value of 1.22 ± 0.05 compared to the control of 2.77 ± 0.2 g. mm. k Pa⁻¹.h⁻¹.m⁻². The monolayer values (M_o) values were 1.063 ± 1.24, 0.6027 ± 1.018, and 0.4100 ± 0.759 g water/g dry film, respectively correspond to H13(50)@CTAB/CMC, H13(100)@CTAB/CMC, and H13(150)@CTAB/CMC films compared with CMC film 1.897 ± 2.149 g water/g dry film. GAB and Smith's models were reasonable in describing the moisture sorption of H13@CTAB/CMC films. The inclusion of H13@CTAB into CMC enhanced thermal stability of H13@CTAB/CMC film. Activaton energy of CMC and H13@CTAB/CMC films were -0.069 and -0.084 J/mole. H13@CTAB/CMC film has broad antimicrobial spectrum action and the most sensitive microorganism is Gram negative bacteria (Escherichia coli).