Dentin biomodification with Er, Cr: YSGG laser prior to conditioning with grape seed, green tea and Salvadora persica extracts

Purpose: This study investigated the effect of Grape seed, green tea, and Salvadora persica extracts on the microtensile bond strength of resin-dentin interfaces laser-treated with Er, Cr: YSGG laser after thermocycling. Methods: Eighty extracted human third molars (n = 80) were used to provide mid-coronal dentin, and assigned into two eight groups (n = 10) according to the laser surface treatment and conditioning extract, four groups laser-treated with Er, Cr: YSGG laser with or without conditioning, and three groups conditioned with extracts only (Grape seed, green tea, and Salvadora persica respectively) and one control group with no laser surface treatment and conditioning. All specimens were subjected to 20,000 thermal cycles. Microtensile bond strength was assessed after thermal cycling. Statistical analysis was performed using one-way ANOVA followed by the Tukey post hoc test for pairwise comparisons. Additionally, P-values < 0.05 were considered statistically significant. Results: Using Grape seed and grape tea as a conditioner increased the bond strength with Er, Cr: YSGG laser surface treatment or without compared to the control group (P < 0.001). However, no significant difference in the bond strength of Salvadora persica groups when compared to the control group (p > 0.05). Therefore, both the laser treatment and conditioning can influence the bond strength. Conclusion: Grape seed and green tea extracts improved the adhesion on laser-treated dentin. Salvadora persica extract demonstrated a bond strength comparable to adhesive when used solely. However, it took advantage of antibacterial, antifungal, anti-inflammatory, antioxidant, analgesic, and anti-cariogenic actions.