Abstract
A densitometric HPTLC method for analysis of cordifolioside A both in 60% methanolic extract of Tinospora cordifolia and in a commercial formulation has been established and validated. Cordifolioside A was separated on aluminum-backed silica gel 60 F254 plates with chloroform-methanol 85:15 (%, v/v) as mobile phase. A compact band was obtained for cordifolioside A at R F 0.52 ± 0.03. The limits of detection (LOD) and quantification (LOQ) were 20.12 and 60.36 ng per band, respectively. The highly precise and accurate method was used for analysis of cordifolioside A.
| Original language | English |
|---|---|
| Pages (from-to) | 683-692 |
| Number of pages | 10 |
| Journal | Acta Chromatographica |
| Volume | 21 |
| Issue number | 4 |
| DOIs | |
| State | Published - 1 Dec 2009 |
| Externally published | Yes |
Keywords
- Cordifolioside A
- HPTLC-densitometry
- ICH guidelines
- Tinospora cordifolia