Correlation between smoking and downregulation of red cell CD47 as eryptosis marker

OBJECTIVE: The purpose of this research is to discover a link between cigarette smoking and decreased red cell CD47 expression. SUBJECTS AND METHODS: The current cross-sectional study included 72 smokers (who had smoked 20 cigarettes per day for at least two years) and 50 nonsmokers, as well as nonsmokers who had not been exposed to smokers on a regular basis and chose to participate as controls. Due to exclusion criteria, 11 participants were removed from the study; they had various genetic, immune, and metabolic disorders, leaving only 61 healthy people in the study. A flow cytometer was used to examine CD47. RESULTS: There was a strong correlation between smoking and a decrease in CD47 markers in all types of smokers in the control samples (p-value = 0.000), as well as among cigarette smokers only (p-value = 0.000), cigarette and Shisha smokers (p-value = 0.024), and cigarette and e-cigarette smokers (p-value = 0.014). Furthermore, there is a strong correlation between the appearance of the CD47 marker in healthy smokers and smokers with non-hereditary blood diseases like iron deficiency anemia and polycythemia. CONCLUSIONS: It can be concluded that smoking significantly reduces the expression of the CD47 marker.