Antimicrobial, antibiofilm and antioxidant activities of<i> Mukia</i><i> maderaspatana</i> (L.) M. Roem. methanolic extract against catheter-associated urinary tract infectious agents

The biofilm related catheter-associated urinary tract infections (CAUTI) are gathering great attention due to severe complication resulting in increased morbidity rate. Therefore, alternative antimicrobials are urgently required for the treatment of CAUTI. Consequently, our study explored the Mukia maderaspatana (L.) M. Roem. antimicrobial and antibiofilm potentials against Escherichia coli, Staphylococcus aureus, Candida albicans and Enterococcus faecalis. The antipathogenic activities were documented against test pathogens and the activity was noted at 0.5 mg/ml against E. coli and S. aureus and also, 1 mg/ml against C. albicans and E. faecalis using microdilution method. The M. maderaspatana antibiofilm activity was examined using crystal violet staining method and effectively inhibited biofilm forming test pathogens. Similarly, 3X MIC of M. maderaspatana extract effectively eliminated the maximum of 86%, 86%, 86%, and 87% of S. aureus, E. coli, E. faecalis, biofilms C. albicans mature biofilms. The M. maderaspatana extract coated catheter tube showed antimicrobial activity against test pathogens. Further, antioxidant property of M. maderaspatana was investigated and cytotoxicity study revealed no toxic effect on normal L929 cells. Based on above results, M. maderaspatana could be a better alternative antimicrobial agent for CAUTI.