TY - JOUR
T1 - Anti-leishmanial activities of extracts and isolated compounds from drechslera rostrata and eurotium tonpholium
AU - Awaad, Amani S.
AU - Al-Zaylaee, Haifa M.
AU - Alqasoumi, Saleh I.
AU - Zain, Mohamed E.
AU - Aloyan, Ebtesam M.
AU - Alafeefy, Ahmed M.
AU - Awad, Elham S.H.
AU - El-Meligy, Reham M.
PY - 2014/5
Y1 - 2014/5
N2 - The fungal extract of Drechslera rostrata and Eurotium tonpholium showed a significant anti-leishmanial activity against Leishmania major; IC50 was 28.8 and 28.2 μg/mL, respectively. Seven compounds, five from D. rostrata (H1-H5) and two from E. tonpholium (H6 and H7), were isolated and identified using different spectroscopic analysis including 1HNMR, 13CNMR, Hetero-nuclear multiple bond connectivity (HMBC), Hetero-nuclear Multiple Quantum Correlation (HMQC), and EI-MS. The isolated compounds are: di-2-ethylhexyl phthalate (1), (22E)-5α,8α- epidioxyergosta-6,22-diene-3β-ol (2),1,3,8-trihydroxy-6-methyl-nthraquinone (3), aloe-emodine 8-O-glucopyranoside(4), 2R, 3R,4R,5R hexane 1, 2, 3, 4, 5, 6 hexole (Mannitol) (5), 1,8-dihydroxy-3-methoxy-6-methyl-anthraquinone (6) and 1, 4, 5-trihydroxy-7-methoxy-2-methyl-anthraquinone (7). However, compounds (1) and (6) showed activity against L. major with IC50 of 3.2 and 10.38 μg/mL, respectively. On the other hand, oral administration of the two extracts (100 mg/kg) and compounds 1 and 6 (50 mg/kg) showed very good activity when compared with the anti-leishmanial drug Pentostam (125 mg/kg). Interestingly, the complete heeling activity of the extracts and compounds (1) and (6) was obtained after 13-17 days of treatment, while complete healing activity of Pentostam was obtained after 28 days. No alteration on liver and kidney functions was recorded on animals treated with the two extracts for 15 consecutive days.
AB - The fungal extract of Drechslera rostrata and Eurotium tonpholium showed a significant anti-leishmanial activity against Leishmania major; IC50 was 28.8 and 28.2 μg/mL, respectively. Seven compounds, five from D. rostrata (H1-H5) and two from E. tonpholium (H6 and H7), were isolated and identified using different spectroscopic analysis including 1HNMR, 13CNMR, Hetero-nuclear multiple bond connectivity (HMBC), Hetero-nuclear Multiple Quantum Correlation (HMQC), and EI-MS. The isolated compounds are: di-2-ethylhexyl phthalate (1), (22E)-5α,8α- epidioxyergosta-6,22-diene-3β-ol (2),1,3,8-trihydroxy-6-methyl-nthraquinone (3), aloe-emodine 8-O-glucopyranoside(4), 2R, 3R,4R,5R hexane 1, 2, 3, 4, 5, 6 hexole (Mannitol) (5), 1,8-dihydroxy-3-methoxy-6-methyl-anthraquinone (6) and 1, 4, 5-trihydroxy-7-methoxy-2-methyl-anthraquinone (7). However, compounds (1) and (6) showed activity against L. major with IC50 of 3.2 and 10.38 μg/mL, respectively. On the other hand, oral administration of the two extracts (100 mg/kg) and compounds 1 and 6 (50 mg/kg) showed very good activity when compared with the anti-leishmanial drug Pentostam (125 mg/kg). Interestingly, the complete heeling activity of the extracts and compounds (1) and (6) was obtained after 13-17 days of treatment, while complete healing activity of Pentostam was obtained after 28 days. No alteration on liver and kidney functions was recorded on animals treated with the two extracts for 15 consecutive days.
KW - 1, 8-dihydroxy-3-methoxy-6-methyl-anthraquinone
KW - 8-peroxide diisooctyl phthalate
KW - anti-leishmanial activity
KW - Drechslera rostrata
KW - ergosterol-5
KW - Eurotium tonpholium
KW - Leishmania major
KW - mannitol
UR - https://www.scopus.com/pages/publications/84899492588
U2 - 10.1002/ptr.5096
DO - 10.1002/ptr.5096
M3 - Article
C2 - 24375822
AN - SCOPUS:84899492588
SN - 0951-418X
VL - 28
SP - 774
EP - 780
JO - Phytotherapy Research
JF - Phytotherapy Research
IS - 5
ER -