TY - JOUR
T1 - Advances in molecular methodologies and nanotechnology-enabled drug delivery in biomedicine
T2 - An overview of current progress
AU - Gacem, Amel
AU - Soltane, Raya
AU - Wanale, Shivraj Gangadhar
AU - Mohammed, Salman Arafath
AU - Almulla, Nuha
AU - Eltayeb, Lienda Bashier
AU - Alreshidi, Maha Awjan
AU - Alasiri, Ahlam
AU - Albakri, Ghadah Shukri
AU - Raghuvanshi, Urmila
AU - Abdulmonem, Waleed Al
AU - Qasim, Maytham T.
AU - Gupta, Rupesh
AU - Islam, Saiful
AU - Yadav, Krishna Kumar
N1 - Publisher Copyright:
© 2025 Elsevier Ltd
PY - 2026/2
Y1 - 2026/2
N2 - Recent advances in molecular techniques and nanosystems have significantly transformed biomedical research, particularly in pathogen detection and drug delivery. Traditional culture-based methods are being increasingly replaced by rapid and precise molecular approaches, offering greater accuracy in identifying pathogens and uncovering the genetic mechanisms underlying antimicrobial resistance (AMR). This review highlights key molecular tools, including matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS), whole-genome sequencing, DNA microarrays, metagenomics, and polymerase chain reaction (PCR) and others with a critical evaluation of their strengths, limitations, and applications in global surveillance. Alongside, the integration of nanostructured systems has opened new avenues for designing advanced drug-delivery platforms with improved bioavailability, targeted action, and reduced toxicity. Together, these innovations emphasize the potential of molecular and nanoscale technologies to address pressing challenges in diagnostics, therapeutics, and the management of infectious diseases.
AB - Recent advances in molecular techniques and nanosystems have significantly transformed biomedical research, particularly in pathogen detection and drug delivery. Traditional culture-based methods are being increasingly replaced by rapid and precise molecular approaches, offering greater accuracy in identifying pathogens and uncovering the genetic mechanisms underlying antimicrobial resistance (AMR). This review highlights key molecular tools, including matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS), whole-genome sequencing, DNA microarrays, metagenomics, and polymerase chain reaction (PCR) and others with a critical evaluation of their strengths, limitations, and applications in global surveillance. Alongside, the integration of nanostructured systems has opened new avenues for designing advanced drug-delivery platforms with improved bioavailability, targeted action, and reduced toxicity. Together, these innovations emphasize the potential of molecular and nanoscale technologies to address pressing challenges in diagnostics, therapeutics, and the management of infectious diseases.
KW - Antibiotic techniques, Biomedical applications
KW - Antimicrobial agents
KW - Drug
KW - Molecular techniques, Nanosystems
UR - https://www.scopus.com/pages/publications/105021856898
U2 - 10.1016/j.compbiolchem.2025.108765
DO - 10.1016/j.compbiolchem.2025.108765
M3 - Review article
AN - SCOPUS:105021856898
SN - 1476-9271
VL - 120
JO - Computational Biology and Chemistry
JF - Computational Biology and Chemistry
M1 - 108765
ER -