Abstract
A high-performance thin layer chromatographic densitometric method for the analysis of swertiamarin in 60% methanolic extract of Enicostemma littorale and commercial formulations has been developed and validated in this study. Swertiamarin was separated on aluminium-backed silica gel 60 F254 plates using ethyl acetate : methanol : water (77 : 15 : 8)% v/v as the mobile phase. This system was found to give a compact spot of swertiamarin at Rf value 0.36±0.01. The limit of detection and limit of quantification were found to be 31.25 and 103.12 ng spot-1, respectively. The proposed method was employed with a high degree of precision and accuracy for the estimation of swertiamarin in methanolic extract of Enicostemma littorale and in commercial formulations.
| Original language | English |
|---|---|
| Pages (from-to) | 17-25 |
| Number of pages | 9 |
| Journal | Natural Product Research |
| Volume | 25 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2011 |
Keywords
- Enicostemma littorale
- HPTLC densitometry
- ICH guidelines
- swertiamarin