TY - JOUR
T1 - A comprehensive appraisal of mechanism of anti-CRISPR proteins
T2 - an advanced genome editor to amend the CRISPR gene editing
AU - Choudhary, Nisha
AU - Tandi, Dipty
AU - Verma, Rakesh Kumar
AU - Yadav, Virendra Kumar
AU - Dhingra, Naveen
AU - Ghosh, Tathagata
AU - Choudhary, Mahima
AU - Gaur, Rajarshi K.
AU - Abdellatif, Magda H.
AU - Gacem, Amel
AU - Eltayeb, Lienda Bashier
AU - Alqahtani, Mohammed S.
AU - Yadav, Krishna Kumar
AU - Jeon, Byong Hun
N1 - Publisher Copyright:
Copyright © 2023 Choudhary, Tandi, Verma, Yadav, Dhingra, Ghosh, Choudhary, Gaur, Abdellatif, Gacem, Eltayeb, Alqahtani, Yadav and Jeon.
PY - 2023
Y1 - 2023
N2 - The development of precise and controlled CRISPR-Cas tools has been made possible by the discovery of protein inhibitors of CRISPR-Cas systems, called anti-CRISPRs (Acrs). The Acr protein has the ability to control off-targeted mutations and impede Cas protein–editing operations. Acr can help with selective breeding, which could help plants and animals improve their valuable features. In this review, the Acr protein–based inhibitory mechanisms that have been adopted by several Acrs, such as (a) the interruption of CRISPR-Cas complex assembly, (b) interference with target DNA binding, (c) blocking of target DNA/RNA cleavage, and (d) enzymatic modification or degradation of signalling molecules, were discussed. In addition, this review emphasizes the applications of Acr proteins in the plant research.
AB - The development of precise and controlled CRISPR-Cas tools has been made possible by the discovery of protein inhibitors of CRISPR-Cas systems, called anti-CRISPRs (Acrs). The Acr protein has the ability to control off-targeted mutations and impede Cas protein–editing operations. Acr can help with selective breeding, which could help plants and animals improve their valuable features. In this review, the Acr protein–based inhibitory mechanisms that have been adopted by several Acrs, such as (a) the interruption of CRISPR-Cas complex assembly, (b) interference with target DNA binding, (c) blocking of target DNA/RNA cleavage, and (d) enzymatic modification or degradation of signalling molecules, were discussed. In addition, this review emphasizes the applications of Acr proteins in the plant research.
KW - Acr inhibitors
KW - anti-CRISPR
KW - CRISPR-Cas complex
KW - DNA binding inhibition
KW - DNA cleavage
KW - genetic memory
KW - genome editing
UR - https://www.scopus.com/pages/publications/85165028218
U2 - 10.3389/fpls.2023.1164461
DO - 10.3389/fpls.2023.1164461
M3 - Review article
AN - SCOPUS:85165028218
SN - 1664-462X
VL - 14
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
M1 - 1164461
ER -